Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system

Claudia Voena; Giovanni Locatelli; Claudia Castellino; Paola Omedè; Marco Ladetto; Elisabetta Zappone; Raffaella Milani; Vittorio Perfetti; Mario Boccadoro; Alessandro Pileri; Paolo Lusso; Chiara Villa; Mauro Malnati; Paolo Corradini

doi:10.1046/j.1365-2141.2002.03448.x

Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34⁺ cells with small- and large-scale Miltenyi cell sorting system

Claudia Voena
, Giovanni Locatelli
, Claudia Castellino
, Paola Omedè
, Marco Ladetto
, Elisabetta Zappone
, Raffaella Milani
, Vittorio Perfetti
, Mario Boccadoro
, Alessandro Pileri
, Paolo Lusso
, Chiara Villa
, Mauro Malnati
, Paolo Corradini

Research output: Contribution to journal › Article › peer-review

Abstract

The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.

Original language	English
Pages (from-to)	642-645
Number of pages	4
Journal	British Journal of Haematology
Volume	117
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-2141.2002.03448.x
Publication status	Published - 2002
Externally published	Yes

Keywords

CD34 cell selection
Immunoglobulin genes
Multiple myeloma
Taqman PCR

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10.1046/j.1365-2141.2002.03448.x

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Voena, C., Locatelli, G., Castellino, C., Omedè, P., Ladetto, M., Zappone, E., Milani, R., Perfetti, V., Boccadoro, M., Pileri, A., Lusso, P., Villa, C., Malnati, M., & Corradini, P. (2002). Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34⁺ cells with small- and large-scale Miltenyi cell sorting system. British Journal of Haematology, 117(3), 642-645. https://doi.org/10.1046/j.1365-2141.2002.03448.x

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title = "Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system",

abstract = "The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.",

keywords = "CD34 cell selection, Immunoglobulin genes, Multiple myeloma, Taqman PCR",

author = "Claudia Voena and Giovanni Locatelli and Claudia Castellino and Paola Omed{\`e} and Marco Ladetto and Elisabetta Zappone and Raffaella Milani and Vittorio Perfetti and Mario Boccadoro and Alessandro Pileri and Paolo Lusso and Chiara Villa and Mauro Malnati and Paolo Corradini",

year = "2002",

doi = "10.1046/j.1365-2141.2002.03448.x",

language = "English",

volume = "117",

pages = "642--645",

journal = "British Journal of Haematology",

issn = "0007-1048",

publisher = "John Wiley and Sons Inc.",

number = "3",

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Voena, C, Locatelli, G, Castellino, C, Omedè, P, Ladetto, M, Zappone, E, Milani, R, Perfetti, V, Boccadoro, M, Pileri, A, Lusso, P, Villa, C, Malnati, M & Corradini, P 2002, 'Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34⁺ cells with small- and large-scale Miltenyi cell sorting system', British Journal of Haematology, vol. 117, no. 3, pp. 642-645. https://doi.org/10.1046/j.1365-2141.2002.03448.x

Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34⁺ cells with small- and large-scale Miltenyi cell sorting system. / Voena, Claudia; Locatelli, Giovanni; Castellino, Claudia et al.
In: British Journal of Haematology, Vol. 117, No. 3, 2002, p. 642-645.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system

AU - Voena, Claudia

AU - Locatelli, Giovanni

AU - Castellino, Claudia

AU - Omedè, Paola

AU - Ladetto, Marco

AU - Zappone, Elisabetta

AU - Milani, Raffaella

AU - Perfetti, Vittorio

AU - Boccadoro, Mario

AU - Pileri, Alessandro

AU - Lusso, Paolo

AU - Villa, Chiara

AU - Malnati, Mauro

AU - Corradini, Paolo

PY - 2002

Y1 - 2002

N2 - The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.

AB - The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.

KW - CD34 cell selection

KW - Immunoglobulin genes

KW - Multiple myeloma

KW - Taqman PCR

UR - https://www.scopus.com/pages/publications/0036113841

U2 - 10.1046/j.1365-2141.2002.03448.x

DO - 10.1046/j.1365-2141.2002.03448.x

M3 - Article

SN - 0007-1048

VL - 117

SP - 642

EP - 645

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 3

ER -

Voena C, Locatelli G, Castellino C, Omedè P, Ladetto M, Zappone E et al. Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34⁺ cells with small- and large-scale Miltenyi cell sorting system. British Journal of Haematology. 2002;117(3):642-645. doi: 10.1046/j.1365-2141.2002.03448.x