Protective Effect of vanilloids against tert-butyl hydroperoxide-induced oxidative stress in vero cells culture

Antonella Rosa, Angela Atzeri, Monica Deiana, M. Paola Melis, Alessandra Incani, Giulia Corona, Debora Loru, Giovanni Appendino, M. Assunta Dessi

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vera cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.

Original languageEnglish
Pages (from-to)3546-3553
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number10
DOIs
Publication statusPublished - 28 May 2008
Externally publishedYes

Keywords

  • Cholesterol
  • Fatty acids
  • Oxidative stress
  • Synthetic capsiate
  • Vanilloids
  • Vanillyl alcohol
  • Vero cells

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