Abstract
This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vera cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.
Original language | English |
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Pages (from-to) | 3546-3553 |
Number of pages | 8 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 56 |
Issue number | 10 |
DOIs | |
Publication status | Published - 28 May 2008 |
Externally published | Yes |
Keywords
- Cholesterol
- Fatty acids
- Oxidative stress
- Synthetic capsiate
- Vanilloids
- Vanillyl alcohol
- Vero cells