Promoter for the human ferritin heavy chain-encoding gene (FERH): structural and functional characterization

M. A. Bevilacqua; M. Giordano; P. D'Agostino; C. Santoro; F. Cimino; F. Costanzo

doi:10.1016/0378-1119(92)90696-M

Promoter for the human ferritin heavy chain-encoding gene (FERH): structural and functional characterization

M. A. Bevilacqua
, M. Giordano
, P. D'Agostino
, C. Santoro
, F. Cimino
, F. Costanzo

Research output: Contribution to journal › Article › peer-review

Abstract

We conducted a functional analysis of the promoter for the human ferritin heavy chain-encoding gene (pFERH) in HepG2 and HeLa cells. The activity of pFERH is equivalent in both cell types, despite their different ferritin (Fer) isotypes. Transfections of a series of 5′-deletion mutants indicate that pFERH activity is essentially dependent on two motifs. One of them, accounting for about 50% of the total transcriptional activity, is recognized by the RNA polymerase II transcription factor, Sp1, and the other by a low-affinity factor present in both the cell types analyzed.

Original language	English
Pages (from-to)	255-260
Number of pages	6
Journal	Gene
Volume	111
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(92)90696-M
Publication status	Published - 15 Feb 1992
Externally published	Yes

Keywords

DNA footprinting
Recombinant DNA
chloramphenicol acetyltransferase assay
gene regulation
transcription factor Sp1

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10.1016/0378-1119(92)90696-M

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title = "Promoter for the human ferritin heavy chain-encoding gene (FERH): structural and functional characterization",

abstract = "We conducted a functional analysis of the promoter for the human ferritin heavy chain-encoding gene (pFERH) in HepG2 and HeLa cells. The activity of pFERH is equivalent in both cell types, despite their different ferritin (Fer) isotypes. Transfections of a series of 5′-deletion mutants indicate that pFERH activity is essentially dependent on two motifs. One of them, accounting for about 50\% of the total transcriptional activity, is recognized by the RNA polymerase II transcription factor, Sp1, and the other by a low-affinity factor present in both the cell types analyzed.",

keywords = "DNA footprinting, Recombinant DNA, chloramphenicol acetyltransferase assay, gene regulation, transcription factor Sp1",

author = "Bevilacqua, \{M. A.\} and M. Giordano and P. D'Agostino and C. Santoro and F. Cimino and F. Costanzo",

note = "Funding Information: The authors deeply thank T. Russo for helpful suggestions and for having read the manuscript, and A.R. Mogavero for skillful technical assistance. The plasmid 2x(Adeno CTF/NF1)SV40CAT was a generous gift from A. Riccio. This work was supported by grants from P.F.ln-gegneria Genetica, CNR (Rome) and from Ministero dell'Universit\textasciitilde{} e della Ricerca Scientifica e Tecnologica (M.U.R.S.T.) (40\textasciitilde{} and 60\textasciitilde{}).",

year = "1992",

month = feb,

day = "15",

doi = "10.1016/0378-1119(92)90696-M",

language = "English",

volume = "111",

pages = "255--260",

journal = "Gene",

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publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Promoter for the human ferritin heavy chain-encoding gene (FERH)

T2 - structural and functional characterization

AU - Bevilacqua, M. A.

AU - Giordano, M.

AU - D'Agostino, P.

AU - Santoro, C.

AU - Cimino, F.

AU - Costanzo, F.

N1 - Funding Information: The authors deeply thank T. Russo for helpful suggestions and for having read the manuscript, and A.R. Mogavero for skillful technical assistance. The plasmid 2x(Adeno CTF/NF1)SV40CAT was a generous gift from A. Riccio. This work was supported by grants from P.F.ln-gegneria Genetica, CNR (Rome) and from Ministero dell'Universit~ e della Ricerca Scientifica e Tecnologica (M.U.R.S.T.) (40~ and 60~).

PY - 1992/2/15

Y1 - 1992/2/15

N2 - We conducted a functional analysis of the promoter for the human ferritin heavy chain-encoding gene (pFERH) in HepG2 and HeLa cells. The activity of pFERH is equivalent in both cell types, despite their different ferritin (Fer) isotypes. Transfections of a series of 5′-deletion mutants indicate that pFERH activity is essentially dependent on two motifs. One of them, accounting for about 50% of the total transcriptional activity, is recognized by the RNA polymerase II transcription factor, Sp1, and the other by a low-affinity factor present in both the cell types analyzed.

AB - We conducted a functional analysis of the promoter for the human ferritin heavy chain-encoding gene (pFERH) in HepG2 and HeLa cells. The activity of pFERH is equivalent in both cell types, despite their different ferritin (Fer) isotypes. Transfections of a series of 5′-deletion mutants indicate that pFERH activity is essentially dependent on two motifs. One of them, accounting for about 50% of the total transcriptional activity, is recognized by the RNA polymerase II transcription factor, Sp1, and the other by a low-affinity factor present in both the cell types analyzed.

KW - DNA footprinting

KW - Recombinant DNA

KW - chloramphenicol acetyltransferase assay

KW - gene regulation

KW - transcription factor Sp1

UR - https://www.scopus.com/pages/publications/0026579434

U2 - 10.1016/0378-1119(92)90696-M

DO - 10.1016/0378-1119(92)90696-M

M3 - Article

SN - 0378-1119

VL - 111

SP - 255

EP - 260

JO - Gene

JF - Gene

IS - 2

ER -

Promoter for the human ferritin heavy chain-encoding gene (FERH): structural and functional characterization

Abstract

Keywords

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