@inbook{40da903080ec4cd19d9fff0f2325d785,
title = "Profiling the autoantibody repertoire by screening phage-displayed human cDNA libraries",
abstract = "The advent of the serological identification of antigens by procedures such as cDNA cloning and recombinant protein expression has allowed the direct molecular definition of immunogenic proteins. The phage-display technology provides several advantages over conventional immunoscreening procedures based on plasmid or lambda-phage cDNA libraries. So far, attempts to display open reading frames, such as those encoded by cDNA fragments, on filamentous phages have not been very successful. We managed to develop a strategy based on {"}folding reporters{"} which allows filtering out open reading frames from DNA and displaying them on filamentous phages in such a way that they are amenable to subsequent selection or screening. Once the cDNA library of interest is created, phage-display technology is used for selection of novel putative antigens; these are then validated by printing isolated protein on microarray and screening with patients' sera.",
keywords = "Cre-recombinase, Phage display, antigens, autoimmunity, high throughput, open reading frames, protein microarray, recombinant fusion protein, serum profiling",
author = "\{Di Niro\}, Roberto and Sara D'Angelo and Paola Secco and Roberto Marzari and Claudio Santoro and Daniele Sblattero",
year = "2009",
doi = "10.1007/978-1-60327-394-7\_20",
language = "English",
isbn = "9781603273930",
series = "Methods in Molecular Biology",
pages = "353--369",
editor = "Marina Cretich and Marcella Chiari",
booktitle = "Peptide Microarrays",
}