Production of ipt-expressing white poplar lines (populus alba l.) with abnormal root morphology

Botti Silvia; Alma Balestrazzi; Daniela Carbonera; Samanta Zelasco; Stefania Biondi; Guido Lingua

doi:10.1080/00087114.2007.10589570

Production of ipt-expressing white poplar lines (populus alba l.) with abnormal root morphology

Botti Silvia
, Alma Balestrazzi
, Daniela Carbonera
, Samanta Zelasco
, Stefania Biondi
, Guido Lingua

Department of Science and Technological Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

Agrobacterium-mediated transformation of white poplar explants was performed using the pMAT-MT construct derived from the ipt-type MAT22 plasmid carrying the PsMT_A1 cDNA encoding a pea metallothionein. In vitro shoot regeneration occurred on antibiotic-free medium and transformants were identified by visual selection. Beside the expected abnormal ipt-shooty phenotype, shoots with normal morphology were recovered. Interestingly, an additional phenotype characterised by a remarkable root development was also observed.

Original language	English
Pages (from-to)	175-177
Number of pages	3
Journal	Caryologia
Volume	60
Issue number	1-2
DOIs	https://doi.org/10.1080/00087114.2007.10589570
Publication status	Published - 1 Jan 2007

Keywords

Ipt gene
Metallothionein
Morphological marker
Phytoremediation
Populus alba

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10.1080/00087114.2007.10589570

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title = "Production of ipt-expressing white poplar lines (populus alba l.) with abnormal root morphology",

abstract = "Agrobacterium-mediated transformation of white poplar explants was performed using the pMAT-MT construct derived from the ipt-type MAT22 plasmid carrying the PsMTA1 cDNA encoding a pea metallothionein. In vitro shoot regeneration occurred on antibiotic-free medium and transformants were identified by visual selection. Beside the expected abnormal ipt-shooty phenotype, shoots with normal morphology were recovered. Interestingly, an additional phenotype characterised by a remarkable root development was also observed.",

keywords = "Ipt gene, Metallothionein, Morphological marker, Phytoremediation, Populus alba",

author = "Botti Silvia and Alma Balestrazzi and Daniela Carbonera and Samanta Zelasco and Stefania Biondi and Guido Lingua",

note = "Funding Information: Acknowledgments — This work was supported by a grant from Ministero dell{\textquoteright}Universit{\`a} e della Ricerca Scientifica (PRIN 2005), Italy.",

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doi = "10.1080/00087114.2007.10589570",

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T1 - Production of ipt-expressing white poplar lines (populus alba l.) with abnormal root morphology

AU - Silvia, Botti

AU - Balestrazzi, Alma

AU - Carbonera, Daniela

AU - Zelasco, Samanta

AU - Biondi, Stefania

AU - Lingua, Guido

N1 - Funding Information: Acknowledgments — This work was supported by a grant from Ministero dell’Università e della Ricerca Scientifica (PRIN 2005), Italy.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Agrobacterium-mediated transformation of white poplar explants was performed using the pMAT-MT construct derived from the ipt-type MAT22 plasmid carrying the PsMTA1 cDNA encoding a pea metallothionein. In vitro shoot regeneration occurred on antibiotic-free medium and transformants were identified by visual selection. Beside the expected abnormal ipt-shooty phenotype, shoots with normal morphology were recovered. Interestingly, an additional phenotype characterised by a remarkable root development was also observed.

AB - Agrobacterium-mediated transformation of white poplar explants was performed using the pMAT-MT construct derived from the ipt-type MAT22 plasmid carrying the PsMTA1 cDNA encoding a pea metallothionein. In vitro shoot regeneration occurred on antibiotic-free medium and transformants were identified by visual selection. Beside the expected abnormal ipt-shooty phenotype, shoots with normal morphology were recovered. Interestingly, an additional phenotype characterised by a remarkable root development was also observed.

KW - Ipt gene

KW - Metallothionein

KW - Morphological marker

KW - Phytoremediation

KW - Populus alba

UR - https://www.scopus.com/pages/publications/65449123216

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DO - 10.1080/00087114.2007.10589570

M3 - Article

SN - 0008-7114

VL - 60

SP - 175

EP - 177

JO - Caryologia

JF - Caryologia

IS - 1-2

ER -

Production of ipt-expressing white poplar lines (populus alba l.) with abnormal root morphology

Abstract

Keywords

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