Polymeric Proanthocyanidins in Skins and Seeds of 37 Vitis vinifera L. Cultivars: A Methodological Comparative Study

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Abstract

The polymeric proanthocyanidins (PAs) composition of skins and seeds from differentVitis viniferaL. cv. was evaluated, sampling 37 cultivar, preserved in the grapevine collection of Grinzane Cavour (Piedmont, Italy). PAs were quantified using both spectrophotometric (n-butanol and vanillin assay) and chromatographic (high-performance liquid chromatography [HPLC]) methods, and the evaluation of their antioxidant activity was performed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Results of PAs quantification with the different methods were significantly correlated. A strong correlation pointed out between anthocyans and PAs content (particularly regarding HPLC method;r= 0.9359,P= 0), and the antioxidant activity was not dependent on the polymeric PAs content, but negatively correlated with the degree of polymerization (r=-0.6102,P= 1 × 10 -4). The hierarchical clustering on principal components analysis allowed grouping the samples in 3 different clusters, observing a direct association between the cluster classification and the anthocyans/PAs content.

Original languageEnglish
Pages (from-to)C742-C749
JournalJournal of Food Science
Volume76
Issue number5
DOIs
Publication statusPublished - Jun 2011

Keywords

  • Grape
  • HPLC
  • Multivariate statistical analysis
  • Proanthocyanidin
  • Spectrophotometric assays

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