Abstract
PM10 was sampled in a background urban site in Torino, a northern Italian city. PM10 extracts were tested with THP-1 and A-549 cells to evaluate their effects on cell proliferation, LDH activity, TNFα, IL8 and CYP1A1 expression, and genotoxic damage induction (Comet assay). Through Principal Component Analysis (PCA), it was observed that (1) the aqueous extracts induced the inhibition of cell proliferation in the warm season that clustered together to total ions, (2) organic extracts determined a winter cell viability reduction and (3) there was a genotoxic effect associated with PAH and metal concentrations. The analysed low PAH levels were unable to induce significant CYP1A1 expression. The results obtained confirmed that PM composition and seasonality play an important role in particle-induced toxicity. The presence of PM10-induced biological effects at a low polluted site suggested that a reduction of PM10 mass did not seem to be sufficient to reduce its toxicity.
Original language | English |
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Pages (from-to) | 833-844 |
Number of pages | 12 |
Journal | Environmental Toxicology and Pharmacology |
Volume | 39 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Mar 2015 |
Keywords
- Air quality
- Genotoxicity
- In vitro toxicology
- PCA
- PM