Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

Chlapanidas Theodora; Perteghella Sara; Faragò Silvio; Boschi Alessandra; Giuseppe Tripodo; Vigani Barbara; Crivelli Barbara; Renzi Sabrina; Dotti Silvia; Preda Stefania; Marazzi Mario; Torre Maria Luisa; Ferrari Maura

doi:10.1002/app.42942

Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

Chlapanidas Theodora, Perteghella Sara, Faragò Silvio, Boschi Alessandra, Giuseppe Tripodo, Vigani Barbara, Crivelli Barbara, Renzi Sabrina, Dotti Silvia, Preda Stefania, Marazzi Mario, Torre Maria Luisa, Ferrari Maura

Research output: Contribution to journal › Article › peer-review

Abstract

In this work platelet lysate (PL) and adipose-derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico-chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing.

Original language	English
Article number	42942
Journal	Journal of Applied Polymer Science
Volume	133
Issue number	5
DOIs	https://doi.org/10.1002/app.42942
Publication status	Published - 1 Feb 2016
Externally published	Yes

Keywords

biomaterials
fibers
properties and characterization

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10.1002/app.42942

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Theodora, C., Sara, P., Silvio, F., Alessandra, B., Tripodo, G., Barbara, V., Barbara, C., Sabrina, R., Silvia, D., Stefania, P., Mario, M., Maria Luisa, T., & Maura, F. (2016). Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing. Journal of Applied Polymer Science, 133(5), Article 42942. https://doi.org/10.1002/app.42942

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title = "Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing",

abstract = "In this work platelet lysate (PL) and adipose-derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico-chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing.",

keywords = "biomaterials, fibers, properties and characterization",

author = "Chlapanidas Theodora and Perteghella Sara and Farag{\`o} Silvio and Boschi Alessandra and Giuseppe Tripodo and Vigani Barbara and Crivelli Barbara and Renzi Sabrina and Dotti Silvia and Preda Stefania and Marazzi Mario and {Maria Luisa}, Torre and Ferrari Maura",

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year = "2016",

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doi = "10.1002/app.42942",

language = "English",

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T1 - Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

AU - Theodora, Chlapanidas

AU - Sara, Perteghella

AU - Silvio, Faragò

AU - Alessandra, Boschi

AU - Tripodo, Giuseppe

AU - Barbara, Vigani

AU - Barbara, Crivelli

AU - Sabrina, Renzi

AU - Silvia, Dotti

AU - Stefania, Preda

AU - Mario, Marazzi

AU - Maria Luisa, Torre

AU - Maura, Ferrari

PY - 2016/2/1

Y1 - 2016/2/1

N2 - In this work platelet lysate (PL) and adipose-derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico-chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing.

AB - In this work platelet lysate (PL) and adipose-derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico-chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing.

KW - biomaterials

KW - fibers

KW - properties and characterization

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U2 - 10.1002/app.42942

DO - 10.1002/app.42942

M3 - Article

SN - 0021-8995

VL - 133

JO - Journal of Applied Polymer Science

JF - Journal of Applied Polymer Science

IS - 5

M1 - 42942

ER -

Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

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