NMR structure of the single QALGGH zinc finger domain from the Arabidopsis thaliana SUPERMAN protein

Carla Isernia, Enrico Bucci, Marilisa Leone, Laura Zaccaro, Paola Di Lello, Giuseppe Digilio, Sabrina Esposito, Michele Saviano, Benedetto Di Blasio, Carlo Pedone, Paolo V. Pedone, Roberto Fattorusso

Research output: Contribution to journalArticlepeer-review

Abstract

Zinc finger domains of the classical type represent the most abundant DNA binding domains in eukaryotic transcription factors. Plant proteins contain from one to four zinc finger domains, which are characterized by high conservation of the sequence QALGGH, shown to be critical for DNA-binding activity The Arabidopsis thaliana SUPERMAN protein, which contains a single QALGGH zinc finger, is necessary for proper spatial development of reproductive floral tissues and has been shown to specifically bind to DNA. Here, we report the synthesis and UV and NMR spectroscopic structural characterization of a 37 amino acid SUPERMAN region complexed to a Zn2+ ion (Zn - SUP37) and present the first high-resolution structure of a classical zinc finger domain from a plant protein. The NMR structure of the SUPERMAN zinc finger domain consists of a very well-defined ββα motif, typical of all other Cys2-His2 zinc fingers structurally characterized. As a consequence, the highly conserved QALGGH sequence is located at the N terminus of the α helix. This region of the domain of animal zinc finger proteins consists of hypervariable residues that are responsible for recognizing the DNA bases. Therefore, we propose a peculiar DNA recognition code for the QALGGH zinc finger domain that includes all or some of the amino acid residues at positions - 1, 2, and 3 (numbered relative to the N terminus of the helix) and possibly others at the C-terminal end of the recognition helix. This study further confirms that the zinc finger domain, though very simple, is an extremely versatile DNA binding motif.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalChemBioChem
Volume4
Issue number2-3
DOIs
Publication statusPublished - 3 Mar 2003
Externally publishedYes

Keywords

  • DNA recognition
  • NMR spectroscopy
  • SUPERMAN
  • Structure elucidation
  • Zinc finger domain

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