NMR as a tool to target protein-protein interactions

Rebecca Del Conte, Daniela Lalli, Paola Turano

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

NMR spectroscopy plays a dual role in projects aimed at targeting protein-protein interactions (PPIs).While it has been extensively validated as an efficient technique for the initial screening and identification of weakly interacting fragments and for subsequently guiding their optimization into molecules with higher affinity and more favorable drug-like properties, it also represents an extremely powerful tool to monitor the formation of protein-protein complexes in solution and to obtain structural information on these adducts. It allows the identification of the protein interfaces and, in some cases, provides intermolecular distance and orientational restraints that lead to the definition of the relative arrangement of the two proteins. In particular, it constitutes the structural technique of choice for studying weak/ transient protein-protein interactions, which represent the natural targets for drug discovery projects addressing PPIs.

Original languageEnglish
Title of host publicationDisruption of Protein-Protein Interfaces
Subtitle of host publicationIn Search of New Inhibitors
PublisherSpringer-Verlag BERLIN-HEIDELBERG
Pages83-111
Number of pages29
Volume9783642379994
ISBN (Electronic)9783642379994
ISBN (Print)3642379982, 9783642379987
DOIs
Publication statusPublished - 1 Mar 2013
Externally publishedYes

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