New polymorphisms in the IL-10 promoter region

S. D'Alfonso; M. Rampi; V. Rolando; M. Giordano; P. Momigliano-Richiardi

doi:10.1038/sj.gene.6363666

New polymorphisms in the IL-10 promoter region

S. D'Alfonso
, M. Rampi
, V. Rolando
, M. Giordano
, P. Momigliano-Richiardi

Department of Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Interleukin-10 (IL-10) is an important immunoregulatory cytokine. We searched for new sequence variations in the 5′ flanking region of the IL-10 gene by denaturing high performance liquid chromatography. A 3996 bp region spanning position -3934 to +61 was amplified in 12 polymerase chain reaction (PCR) fragments and each fragment was screened for variations in 23 Italian individuals. The following eight sequence variations all consisting of single base pair substitutions were identified: -3533A/T, -2769A/G, -2739A/G, -2013A/G, -1349A/G, -1255CfT, -851A/G, -657A/G. The new polymorphisms were analysed in an additional panel of random Italian individuals. The same samples were also tested for the IL10.G and IL10.R microsatellites, and for the two previously described single nucleotide polymorphisms (SNPs) at positions -1082 and -592. Highly significant pairwise linkage disequilibria were observed between alleles at most SNPs. Three major haplotypic combinations of alleles at multiple SNP sites were observed.

Original language	English
Pages (from-to)	231-233
Number of pages	3
Journal	Genes and Immunity
Volume	1
Issue number	3
DOIs	https://doi.org/10.1038/sj.gene.6363666
Publication status	Published - Feb 2000

Keywords

DHPLC
IL-10 promoter
Italian population
Polymorphisms

Access to Document

10.1038/sj.gene.6363666

Cite this

@article{e4ecc54a712e4dedafbd98f12df6bb17,

title = "New polymorphisms in the IL-10 promoter region",

abstract = "Interleukin-10 (IL-10) is an important immunoregulatory cytokine. We searched for new sequence variations in the 5′ flanking region of the IL-10 gene by denaturing high performance liquid chromatography. A 3996 bp region spanning position -3934 to +61 was amplified in 12 polymerase chain reaction (PCR) fragments and each fragment was screened for variations in 23 Italian individuals. The following eight sequence variations all consisting of single base pair substitutions were identified: -3533A/T, -2769A/G, -2739A/G, -2013A/G, -1349A/G, -1255CfT, -851A/G, -657A/G. The new polymorphisms were analysed in an additional panel of random Italian individuals. The same samples were also tested for the IL10.G and IL10.R microsatellites, and for the two previously described single nucleotide polymorphisms (SNPs) at positions -1082 and -592. Highly significant pairwise linkage disequilibria were observed between alleles at most SNPs. Three major haplotypic combinations of alleles at multiple SNP sites were observed.",

keywords = "DHPLC, IL-10 promoter, Italian population, Polymorphisms",

author = "S. D'Alfonso and M. Rampi and V. Rolando and M. Giordano and P. Momigliano-Richiardi",

note = "Funding Information: This work was supported by Telethon, grant E162 and by MURST 60\%.",

year = "2000",

month = feb,

doi = "10.1038/sj.gene.6363666",

language = "English",

volume = "1",

pages = "231--233",

journal = "Genes and Immunity",

issn = "1466-4879",

publisher = "Springer Nature",

number = "3",

}

TY - JOUR

T1 - New polymorphisms in the IL-10 promoter region

AU - D'Alfonso, S.

AU - Rampi, M.

AU - Rolando, V.

AU - Giordano, M.

AU - Momigliano-Richiardi, P.

N1 - Funding Information: This work was supported by Telethon, grant E162 and by MURST 60%.

PY - 2000/2

Y1 - 2000/2

N2 - Interleukin-10 (IL-10) is an important immunoregulatory cytokine. We searched for new sequence variations in the 5′ flanking region of the IL-10 gene by denaturing high performance liquid chromatography. A 3996 bp region spanning position -3934 to +61 was amplified in 12 polymerase chain reaction (PCR) fragments and each fragment was screened for variations in 23 Italian individuals. The following eight sequence variations all consisting of single base pair substitutions were identified: -3533A/T, -2769A/G, -2739A/G, -2013A/G, -1349A/G, -1255CfT, -851A/G, -657A/G. The new polymorphisms were analysed in an additional panel of random Italian individuals. The same samples were also tested for the IL10.G and IL10.R microsatellites, and for the two previously described single nucleotide polymorphisms (SNPs) at positions -1082 and -592. Highly significant pairwise linkage disequilibria were observed between alleles at most SNPs. Three major haplotypic combinations of alleles at multiple SNP sites were observed.

AB - Interleukin-10 (IL-10) is an important immunoregulatory cytokine. We searched for new sequence variations in the 5′ flanking region of the IL-10 gene by denaturing high performance liquid chromatography. A 3996 bp region spanning position -3934 to +61 was amplified in 12 polymerase chain reaction (PCR) fragments and each fragment was screened for variations in 23 Italian individuals. The following eight sequence variations all consisting of single base pair substitutions were identified: -3533A/T, -2769A/G, -2739A/G, -2013A/G, -1349A/G, -1255CfT, -851A/G, -657A/G. The new polymorphisms were analysed in an additional panel of random Italian individuals. The same samples were also tested for the IL10.G and IL10.R microsatellites, and for the two previously described single nucleotide polymorphisms (SNPs) at positions -1082 and -592. Highly significant pairwise linkage disequilibria were observed between alleles at most SNPs. Three major haplotypic combinations of alleles at multiple SNP sites were observed.

KW - DHPLC

KW - IL-10 promoter

KW - Italian population

KW - Polymorphisms

UR - https://www.scopus.com/pages/publications/0034139497

U2 - 10.1038/sj.gene.6363666

DO - 10.1038/sj.gene.6363666

M3 - Article

SN - 1466-4879

VL - 1

SP - 231

EP - 233

JO - Genes and Immunity

JF - Genes and Immunity

IS - 3

ER -

New polymorphisms in the IL-10 promoter region

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this