Mouse Satellite Cell Isolation and Transplantation

ELIA ANGELINO, Simone Reano, Michele Ferrara, Emanuela Agosti, Hana Sustova, VALERIA MALACARNE, Sara Clerici, Andrea Graziani, Nicoletta FILIGHEDDU

Research output: Contribution to journalArticlepeer-review

Abstract

: Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).
Original languageEnglish
Pages (from-to)e2696-N/A
Number of pages13
JournalBio-protocol
Volume8
Issue number2
DOIs
Publication statusPublished - 2018

Keywords

  • Cardiotoxin
  • Engraftment
  • Muscle excision
  • Muscle stem cells
  • Regeneration
  • Satellite cells
  • Skeletal muscle
  • Transplant

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