Mechanisms of thrombin-induced modifications of human platelet cytoskeleton

F. Sinigaglia; C. L. Balduini; A. Bisio; C. Balduini

doi:10.1042/bj2320305

Mechanisms of thrombin-induced modifications of human platelet cytoskeleton

F. Sinigaglia
, C. L. Balduini
, A. Bisio
, C. Balduini

Research output: Contribution to journal › Article › peer-review

Abstract

1. Thrombin treated with phenylmethanesulphonyl fluoride, like active enzyme, promotes modifications to human platelet cytoskeleton. 2. The removal of active thrombin by hirudin partially reverses this process. 3. Chymotrypsin-treated platelets do not modify their cytoskeleton after thrombin stimulus, but are still able to increase their adhesiveness to collagen. It is concluded that thrombin influences the cytoskeleton and adhesion by non-enzymic mechanisms which may be mediated by different modulators.

Original language	English
Pages (from-to)	305-308
Number of pages	4
Journal	Biochemical Journal
Volume	232
Issue number	1
DOIs	https://doi.org/10.1042/bj2320305
Publication status	Published - 1985
Externally published	Yes

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title = "Mechanisms of thrombin-induced modifications of human platelet cytoskeleton",

abstract = "1. Thrombin treated with phenylmethanesulphonyl fluoride, like active enzyme, promotes modifications to human platelet cytoskeleton. 2. The removal of active thrombin by hirudin partially reverses this process. 3. Chymotrypsin-treated platelets do not modify their cytoskeleton after thrombin stimulus, but are still able to increase their adhesiveness to collagen. It is concluded that thrombin influences the cytoskeleton and adhesion by non-enzymic mechanisms which may be mediated by different modulators.",

author = "F. Sinigaglia and Balduini, \{C. L.\} and A. Bisio and C. Balduini",

year = "1985",

doi = "10.1042/bj2320305",

language = "English",

volume = "232",

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journal = "Biochemical Journal",

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AU - Sinigaglia, F.

AU - Balduini, C. L.

AU - Bisio, A.

AU - Balduini, C.

PY - 1985

Y1 - 1985

N2 - 1. Thrombin treated with phenylmethanesulphonyl fluoride, like active enzyme, promotes modifications to human platelet cytoskeleton. 2. The removal of active thrombin by hirudin partially reverses this process. 3. Chymotrypsin-treated platelets do not modify their cytoskeleton after thrombin stimulus, but are still able to increase their adhesiveness to collagen. It is concluded that thrombin influences the cytoskeleton and adhesion by non-enzymic mechanisms which may be mediated by different modulators.

AB - 1. Thrombin treated with phenylmethanesulphonyl fluoride, like active enzyme, promotes modifications to human platelet cytoskeleton. 2. The removal of active thrombin by hirudin partially reverses this process. 3. Chymotrypsin-treated platelets do not modify their cytoskeleton after thrombin stimulus, but are still able to increase their adhesiveness to collagen. It is concluded that thrombin influences the cytoskeleton and adhesion by non-enzymic mechanisms which may be mediated by different modulators.

UR - https://www.scopus.com/pages/publications/0022338602

U2 - 10.1042/bj2320305

DO - 10.1042/bj2320305

M3 - Article

SN - 0264-6021

VL - 232

SP - 305

EP - 308

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -

Mechanisms of thrombin-induced modifications of human platelet cytoskeleton

Abstract

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