Isolation of stromal stem cells from adipose tissue

Maria Prat; Francesca Oltolina; Silvia Antonini; Andrea Zamperone

doi:10.1007/978-1-4939-6756-8_13

Isolation of stromal stem cells from adipose tissue

Maria Prat
, Francesca Oltolina
, Silvia Antonini
, Andrea Zamperone

Department of Health Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	169-182
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-4939-6756-8_13
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1553
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Adipose tissue
Cell culturing
Cell differentiation
Cell isolation
Immunophenotyping
Mesenchymal stem cells
Stromal stem cells

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10.1007/978-1-4939-6756-8_13

Cite this

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title = "Isolation of stromal stem cells from adipose tissue",

abstract = "Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium.",

keywords = "Adipose tissue, Cell culturing, Cell differentiation, Cell isolation, Immunophenotyping, Mesenchymal stem cells, Stromal stem cells",

author = "Maria Prat and Francesca Oltolina and Silvia Antonini and Andrea Zamperone",

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T1 - Isolation of stromal stem cells from adipose tissue

AU - Prat, Maria

AU - Oltolina, Francesca

AU - Antonini, Silvia

AU - Zamperone, Andrea

N1 - Publisher Copyright: © Springer Science+Business Media LLC 2017.

PY - 2017

Y1 - 2017

N2 - Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium.

AB - Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium.

KW - Adipose tissue

KW - Cell culturing

KW - Cell differentiation

KW - Cell isolation

KW - Immunophenotyping

KW - Mesenchymal stem cells

KW - Stromal stem cells

UR - https://www.scopus.com/pages/publications/85013923560

U2 - 10.1007/978-1-4939-6756-8_13

DO - 10.1007/978-1-4939-6756-8_13

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 169

EP - 182

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Isolation of stromal stem cells from adipose tissue

Abstract

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UN SDGs

Keywords

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