Investigation by experimental design and regression models of the effect of five experimental factors on ion-interaction high-performance liquid chromatographic retention

E. Marengo; M. C. Gennaro; C. Abrigo

doi:10.1016/0003-2670(95)00553-6

Investigation by experimental design and regression models of the effect of five experimental factors on ion-interaction high-performance liquid chromatographic retention

E. Marengo, M. C. Gennaro, C. Abrigo

Research output: Contribution to journal › Article › peer-review

Abstract

The effect on retention of simazine and atrazine of five experimental factors in ion-interaction chromatography is investigated by means of chemometrics. The factors are: (1) the chainlength of the ion-interaction reagent (N), (2) the concentration of the ion-interaction reagent (CR), (3) the pH of the mobile phase, (4) the now rate (F) and (5) the organic modifier concentration (CM). Fractional and star experimental designs are employed. The effects of the factors and their interaction on retention are calculated and discussed: the results permit to draw out useful information concerning the interaction mechanisms involved. A regression model correlating the chromatographic retention to the factors, their second-order interactions and their squares with very good predictive ability (R² > 97% and R(cv)² > 94%) is also calculated.

Original language	English
Pages (from-to)	225-236
Number of pages	12
Journal	Analytica Chimica Acta
Volume	321
Issue number	2-3
DOIs	https://doi.org/10.1016/0003-2670(95)00553-6
Publication status	Published - 20 Mar 1996
Externally published	Yes

Keywords

Chemometrics
Experimental design
High-performance liquid chromatography
Regression model

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10.1016/0003-2670(95)00553-6

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@article{b5609fe08e034558baf3d363831f5cd8,

title = "Investigation by experimental design and regression models of the effect of five experimental factors on ion-interaction high-performance liquid chromatographic retention",

abstract = "The effect on retention of simazine and atrazine of five experimental factors in ion-interaction chromatography is investigated by means of chemometrics. The factors are: (1) the chainlength of the ion-interaction reagent (N), (2) the concentration of the ion-interaction reagent (CR), (3) the pH of the mobile phase, (4) the now rate (F) and (5) the organic modifier concentration (CM). Fractional and star experimental designs are employed. The effects of the factors and their interaction on retention are calculated and discussed: the results permit to draw out useful information concerning the interaction mechanisms involved. A regression model correlating the chromatographic retention to the factors, their second-order interactions and their squares with very good predictive ability (R2 > 97\% and R(cv)2 > 94\%) is also calculated.",

keywords = "Chemometrics, Experimental design, High-performance liquid chromatography, Regression model",

author = "E. Marengo and Gennaro, \{M. C.\} and C. Abrigo",

note = "Funding Information: The authors gratefully acknowledge financial support by CNR (Consiglio Nazionale delle Ricerche, Rome) and MURST (Ministero dell'Universitlt e della Ricerea Scientifica e Tecnologica, Rome).",

year = "1996",

month = mar,

day = "20",

doi = "10.1016/0003-2670(95)00553-6",

language = "English",

volume = "321",

pages = "225--236",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier B.V.",

number = "2-3",

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TY - JOUR

T1 - Investigation by experimental design and regression models of the effect of five experimental factors on ion-interaction high-performance liquid chromatographic retention

AU - Marengo, E.

AU - Gennaro, M. C.

AU - Abrigo, C.

N1 - Funding Information: The authors gratefully acknowledge financial support by CNR (Consiglio Nazionale delle Ricerche, Rome) and MURST (Ministero dell'Universitlt e della Ricerea Scientifica e Tecnologica, Rome).

PY - 1996/3/20

Y1 - 1996/3/20

N2 - The effect on retention of simazine and atrazine of five experimental factors in ion-interaction chromatography is investigated by means of chemometrics. The factors are: (1) the chainlength of the ion-interaction reagent (N), (2) the concentration of the ion-interaction reagent (CR), (3) the pH of the mobile phase, (4) the now rate (F) and (5) the organic modifier concentration (CM). Fractional and star experimental designs are employed. The effects of the factors and their interaction on retention are calculated and discussed: the results permit to draw out useful information concerning the interaction mechanisms involved. A regression model correlating the chromatographic retention to the factors, their second-order interactions and their squares with very good predictive ability (R2 > 97% and R(cv)2 > 94%) is also calculated.

AB - The effect on retention of simazine and atrazine of five experimental factors in ion-interaction chromatography is investigated by means of chemometrics. The factors are: (1) the chainlength of the ion-interaction reagent (N), (2) the concentration of the ion-interaction reagent (CR), (3) the pH of the mobile phase, (4) the now rate (F) and (5) the organic modifier concentration (CM). Fractional and star experimental designs are employed. The effects of the factors and their interaction on retention are calculated and discussed: the results permit to draw out useful information concerning the interaction mechanisms involved. A regression model correlating the chromatographic retention to the factors, their second-order interactions and their squares with very good predictive ability (R2 > 97% and R(cv)2 > 94%) is also calculated.

KW - Chemometrics

KW - Experimental design

KW - High-performance liquid chromatography

KW - Regression model

UR - https://www.scopus.com/pages/publications/0029983133

U2 - 10.1016/0003-2670(95)00553-6

DO - 10.1016/0003-2670(95)00553-6

M3 - Article

SN - 0003-2670

VL - 321

SP - 225

EP - 236

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2-3

ER -

Investigation by experimental design and regression models of the effect of five experimental factors on ion-interaction high-performance liquid chromatographic retention

Abstract

Keywords

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