Interrelation of platelet aggregation, release reaction and thromboxane A2 production

Carlo L. Balduini; Giampiera Bertolino; Patrizia Noris; Fabiola Sinigaglia; Antonella Bisio; Mauro Torti

doi:10.1016/S0006-291X(88)80918-5

Interrelation of platelet aggregation, release reaction and thromboxane A₂ production

Carlo L. Balduini, Giampiera Bertolino, Patrizia Noris, Fabiola Sinigaglia, Antonella Bisio, Mauro Torti

Research output: Contribution to journal › Article › peer-review

Abstract

Aggregation of platelets, stimulated by different agonists, was inhibited by omitting sample stirring or by preincubation of platelets with a monoclonal antibody against glycoproteins IIb-IIIa or with a pentapeptide containing the sequence Arg-Gly-Asp-Ser. In platelets stimulated by collagen, ADP and epinephrine, the inhibition of aggregation paralleled a reduction of both release reaction and thromboxane A₂ formation. When thrombin was the stimulus, ATP release and thromboxane A₂ production were unaffected (or only slightly modified) by the inhibition of platelet aggregation. These data add further evidence to the hypothesis that aggregation supports the activation of platelets stimulated by weak agonists.

Original language	English
Pages (from-to)	822-829
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	156
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(88)80918-5
Publication status	Published - 31 Oct 1988
Externally published	Yes

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title = "Interrelation of platelet aggregation, release reaction and thromboxane A2 production",

abstract = "Aggregation of platelets, stimulated by different agonists, was inhibited by omitting sample stirring or by preincubation of platelets with a monoclonal antibody against glycoproteins IIb-IIIa or with a pentapeptide containing the sequence Arg-Gly-Asp-Ser. In platelets stimulated by collagen, ADP and epinephrine, the inhibition of aggregation paralleled a reduction of both release reaction and thromboxane A2 formation. When thrombin was the stimulus, ATP release and thromboxane A2 production were unaffected (or only slightly modified) by the inhibition of platelet aggregation. These data add further evidence to the hypothesis that aggregation supports the activation of platelets stimulated by weak agonists.",

author = "Balduini, {Carlo L.} and Giampiera Bertolino and Patrizia Noris and Fabiola Sinigaglia and Antonella Bisio and Mauro Torti",

note = "Funding Information: ACKNOWLEDGMENTS: This work was supported by grants from MPl and CNR, Italy. We thank prof. Damiani (Institute of Biochemistry, Genova, Italy) for the gift of the monoclonal antibody PBM 6.4.",

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T1 - Interrelation of platelet aggregation, release reaction and thromboxane A2 production

AU - Balduini, Carlo L.

AU - Bertolino, Giampiera

AU - Noris, Patrizia

AU - Sinigaglia, Fabiola

AU - Bisio, Antonella

AU - Torti, Mauro

N1 - Funding Information: ACKNOWLEDGMENTS: This work was supported by grants from MPl and CNR, Italy. We thank prof. Damiani (Institute of Biochemistry, Genova, Italy) for the gift of the monoclonal antibody PBM 6.4.

PY - 1988/10/31

Y1 - 1988/10/31

N2 - Aggregation of platelets, stimulated by different agonists, was inhibited by omitting sample stirring or by preincubation of platelets with a monoclonal antibody against glycoproteins IIb-IIIa or with a pentapeptide containing the sequence Arg-Gly-Asp-Ser. In platelets stimulated by collagen, ADP and epinephrine, the inhibition of aggregation paralleled a reduction of both release reaction and thromboxane A2 formation. When thrombin was the stimulus, ATP release and thromboxane A2 production were unaffected (or only slightly modified) by the inhibition of platelet aggregation. These data add further evidence to the hypothesis that aggregation supports the activation of platelets stimulated by weak agonists.

AB - Aggregation of platelets, stimulated by different agonists, was inhibited by omitting sample stirring or by preincubation of platelets with a monoclonal antibody against glycoproteins IIb-IIIa or with a pentapeptide containing the sequence Arg-Gly-Asp-Ser. In platelets stimulated by collagen, ADP and epinephrine, the inhibition of aggregation paralleled a reduction of both release reaction and thromboxane A2 formation. When thrombin was the stimulus, ATP release and thromboxane A2 production were unaffected (or only slightly modified) by the inhibition of platelet aggregation. These data add further evidence to the hypothesis that aggregation supports the activation of platelets stimulated by weak agonists.

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JO - Biochemical and Biophysical Research Communications

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Interrelation of platelet aggregation, release reaction and thromboxane A₂ production

Abstract

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