Inhibitory effect of the somatostatin analog octreotide on rat pituitary tumor cell (GH3) proliferation in vitro

G. Pelicci; M. C. Pagliacci; L. Lanfrancone; P. G. Pelicci; F. Grignani; Ildo Nicoletti

doi:10.1007/BF03349589

Inhibitory effect of the somatostatin analog octreotide on rat pituitary tumor cell (GH₃) proliferation in vitro

G. Pelicci, M. C. Pagliacci, L. Lanfrancone, P. G. Pelicci, F. Grignani, Ildo Nicoletti

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of somatostatin-14 (SS-14) and the somatostatin-analog octreotide (SMS 201-995, Sandostatin) on proliferation of GH₃ pituitary tumor cells were investigated in vitro. SMS 201-995 exerted a significant, but transient, inhibition on GH₃ cell growth which reached a maximum at 24 h and was no longer detectable at 48 h. The concentration that evoked the strongest inhibitory effect was 10 nM SMS 201-995, while lower and higher doses resulted in a less pronounced effect. The inhibitory effect SMS 201-995 exerted on cell proliferation was associated with a dose- and time-related reduction in both c-myc and c-fos mRNA levels. SS-14 had no noteworthy influence on either cell proliferation or c-myc and c-fos protooncogene expression. These data demonstrate that SS-analogs transiently inhibit pituitary tumor cell proliferation in vitro.

Original language	English
Pages (from-to)	657-662
Number of pages	6
Journal	Journal of Endocrinological Investigation
Volume	13
Issue number	8
DOIs	https://doi.org/10.1007/BF03349589
Publication status	Published - Sept 1990
Externally published	Yes

Keywords

GH cells
cell proliferation
octreoide
protooncogenes
somatostatin

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Access to Document

10.1007/BF03349589

Cite this

@article{33a1c19510844cbd98546d38a7b3f358,

title = "Inhibitory effect of the somatostatin analog octreotide on rat pituitary tumor cell (GH3) proliferation in vitro",

abstract = "The effects of somatostatin-14 (SS-14) and the somatostatin-analog octreotide (SMS 201-995, Sandostatin) on proliferation of GH3 pituitary tumor cells were investigated in vitro. SMS 201-995 exerted a significant, but transient, inhibition on GH3 cell growth which reached a maximum at 24 h and was no longer detectable at 48 h. The concentration that evoked the strongest inhibitory effect was 10 nM SMS 201-995, while lower and higher doses resulted in a less pronounced effect. The inhibitory effect SMS 201-995 exerted on cell proliferation was associated with a dose- and time-related reduction in both c-myc and c-fos mRNA levels. SS-14 had no noteworthy influence on either cell proliferation or c-myc and c-fos protooncogene expression. These data demonstrate that SS-analogs transiently inhibit pituitary tumor cell proliferation in vitro.",

keywords = "GH cells, cell proliferation, octreoide, protooncogenes, somatostatin",

author = "G. Pelicci and Pagliacci, {M. C.} and L. Lanfrancone and Pelicci, {P. G.} and F. Grignani and Ildo Nicoletti",

year = "1990",

month = sep,

doi = "10.1007/BF03349589",

language = "English",

volume = "13",

pages = "657--662",

journal = "Journal of Endocrinological Investigation",

issn = "0391-4097",

publisher = "Springer Science and Business Media Deutschland GmbH",

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TY - JOUR

T1 - Inhibitory effect of the somatostatin analog octreotide on rat pituitary tumor cell (GH3) proliferation in vitro

AU - Pelicci, G.

AU - Pagliacci, M. C.

AU - Lanfrancone, L.

AU - Pelicci, P. G.

AU - Grignani, F.

AU - Nicoletti, Ildo

PY - 1990/9

Y1 - 1990/9

N2 - The effects of somatostatin-14 (SS-14) and the somatostatin-analog octreotide (SMS 201-995, Sandostatin) on proliferation of GH3 pituitary tumor cells were investigated in vitro. SMS 201-995 exerted a significant, but transient, inhibition on GH3 cell growth which reached a maximum at 24 h and was no longer detectable at 48 h. The concentration that evoked the strongest inhibitory effect was 10 nM SMS 201-995, while lower and higher doses resulted in a less pronounced effect. The inhibitory effect SMS 201-995 exerted on cell proliferation was associated with a dose- and time-related reduction in both c-myc and c-fos mRNA levels. SS-14 had no noteworthy influence on either cell proliferation or c-myc and c-fos protooncogene expression. These data demonstrate that SS-analogs transiently inhibit pituitary tumor cell proliferation in vitro.

AB - The effects of somatostatin-14 (SS-14) and the somatostatin-analog octreotide (SMS 201-995, Sandostatin) on proliferation of GH3 pituitary tumor cells were investigated in vitro. SMS 201-995 exerted a significant, but transient, inhibition on GH3 cell growth which reached a maximum at 24 h and was no longer detectable at 48 h. The concentration that evoked the strongest inhibitory effect was 10 nM SMS 201-995, while lower and higher doses resulted in a less pronounced effect. The inhibitory effect SMS 201-995 exerted on cell proliferation was associated with a dose- and time-related reduction in both c-myc and c-fos mRNA levels. SS-14 had no noteworthy influence on either cell proliferation or c-myc and c-fos protooncogene expression. These data demonstrate that SS-analogs transiently inhibit pituitary tumor cell proliferation in vitro.

KW - GH cells

KW - cell proliferation

KW - octreoide

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KW - somatostatin

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