In vitro evidence for CCl4 metabolites covalently bound to lipoprotein micelles

G. Poli; E. Albano; F. Biasi; E. Chiarpotto; M. U. Dianzani

doi:10.1016/0014-5793(83)80963-6

In vitro evidence for CCl₄ metabolites covalently bound to lipoprotein micelles

G. Poli
, E. Albano
, F. Biasi
, E. Chiarpotto
, M. U. Dianzani

Research output: Contribution to journal › Article › peer-review

Abstract

CCl₄-induced impairment of the lipoprotein secretion pathway of intact rat hepatocytes was carried out using ¹⁴CCl₄ to check the possibility of binding to lipoproteins by CCl₄ metabolites. After separation of different cell suspension fractions by means of ultracentrifugation and chemical precipitation procedures, a significant amount of the radioisotope was found covalently bound to the lipid and protein components of low density lipoproteins. Suitable experiments demonstrated that the bound radioisotope was represented by CCl₄ metabolites and not by unactivated CCl₄.

Original language	English
Pages (from-to)	187-190
Number of pages	4
Journal	FEBS Letters
Volume	160
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(83)80963-6
Publication status	Published - 22 Aug 1983
Externally published	Yes

Keywords

Carbon tetrachloride
Covalent binding
Fatty liver
Isolated hepatocyte
Lipoprotein secretion
Trichloromethyl radical

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10.1016/0014-5793(83)80963-6

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title = "In vitro evidence for CCl4 metabolites covalently bound to lipoprotein micelles",

abstract = "CCl4-induced impairment of the lipoprotein secretion pathway of intact rat hepatocytes was carried out using 14CCl4 to check the possibility of binding to lipoproteins by CCl4 metabolites. After separation of different cell suspension fractions by means of ultracentrifugation and chemical precipitation procedures, a significant amount of the radioisotope was found covalently bound to the lipid and protein components of low density lipoproteins. Suitable experiments demonstrated that the bound radioisotope was represented by CCl4 metabolites and not by unactivated CCl4.",

keywords = "Carbon tetrachloride, Covalent binding, Fatty liver, Isolated hepatocyte, Lipoprotein secretion, Trichloromethyl radical",

author = "G. Poli and E. Albano and F. Biasi and E. Chiarpotto and Dianzani, \{M. U.\}",

note = "Funding Information: The authorsw ish to thank the National Foundation for Cancer Research, Bethesda,M aryland, and the ConsiglioN azionaled elleR icerche,R ome, Italy, for supportingt his research.",

year = "1983",

month = aug,

day = "22",

doi = "10.1016/0014-5793(83)80963-6",

language = "English",

volume = "160",

pages = "187--190",

journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - In vitro evidence for CCl4 metabolites covalently bound to lipoprotein micelles

AU - Poli, G.

AU - Albano, E.

AU - Biasi, F.

AU - Chiarpotto, E.

AU - Dianzani, M. U.

N1 - Funding Information: The authorsw ish to thank the National Foundation for Cancer Research, Bethesda,M aryland, and the ConsiglioN azionaled elleR icerche,R ome, Italy, for supportingt his research.

PY - 1983/8/22

Y1 - 1983/8/22

N2 - CCl4-induced impairment of the lipoprotein secretion pathway of intact rat hepatocytes was carried out using 14CCl4 to check the possibility of binding to lipoproteins by CCl4 metabolites. After separation of different cell suspension fractions by means of ultracentrifugation and chemical precipitation procedures, a significant amount of the radioisotope was found covalently bound to the lipid and protein components of low density lipoproteins. Suitable experiments demonstrated that the bound radioisotope was represented by CCl4 metabolites and not by unactivated CCl4.

AB - CCl4-induced impairment of the lipoprotein secretion pathway of intact rat hepatocytes was carried out using 14CCl4 to check the possibility of binding to lipoproteins by CCl4 metabolites. After separation of different cell suspension fractions by means of ultracentrifugation and chemical precipitation procedures, a significant amount of the radioisotope was found covalently bound to the lipid and protein components of low density lipoproteins. Suitable experiments demonstrated that the bound radioisotope was represented by CCl4 metabolites and not by unactivated CCl4.

KW - Carbon tetrachloride

KW - Covalent binding

KW - Fatty liver

KW - Isolated hepatocyte

KW - Lipoprotein secretion

KW - Trichloromethyl radical

UR - https://www.scopus.com/pages/publications/0020642980

U2 - 10.1016/0014-5793(83)80963-6

DO - 10.1016/0014-5793(83)80963-6

M3 - Article

SN - 0014-5793

VL - 160

SP - 187

EP - 190

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

In vitro evidence for CCl₄ metabolites covalently bound to lipoprotein micelles

Abstract

Keywords

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