Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells

Rosanna Mezzapelle; Eltjona Rrapaj; Elena Gatti; Chiara Ceriotti; Francesco De Marchis; Alessandro Preti; Antonello E. Spinelli; Laura Perani; Massimo Venturini; Silvia Valtorta; Rosa Maria Moresco; Lorenza Pecciarini; Claudio Doglioni; Michela Frenquelli; Luca Crippa; Camilla Recordati; Eugenio Scanziani; Hilda De Vries; Anton Berns; Roberta Frapolli; Renzo Boldorini; Maurizio D'Incalci; Marco E. Bianchi; Massimo P. Crippa

doi:10.1038/srep22850

Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells

Rosanna Mezzapelle
, Eltjona Rrapaj
, Elena Gatti
, Chiara Ceriotti
, Francesco De Marchis
, Alessandro Preti
, Antonello E. Spinelli
, Laura Perani
, Massimo Venturini
, Silvia Valtorta
, Rosa Maria Moresco
, Lorenza Pecciarini
, Claudio Doglioni
, Michela Frenquelli
, Luca Crippa
, Camilla Recordati
, Eugenio Scanziani
, Hilda De Vries
, Anton Berns
, Roberta Frapolli

Renzo Boldorini, Maurizio D'Incalci, Marco E. Bianchi, Massimo P. Crippa

Research output: Contribution to journal › Article › peer-review

Abstract

Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment.

Original language	English
Article number	22850
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep22850
Publication status	Published - 10 Mar 2016
Externally published	Yes

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SDG 3 Good Health and Well-being

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10.1038/srep22850

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Mezzapelle, R., Rrapaj, E., Gatti, E., Ceriotti, C., De Marchis, F., Preti, A., Spinelli, A. E., Perani, L., Venturini, M., Valtorta, S., Moresco, R. M., Pecciarini, L., Doglioni, C., Frenquelli, M., Crippa, L., Recordati, C., Scanziani, E., De Vries, H., Berns, A., ... Crippa, M. P. (2016). Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells. Scientific Reports, 6, Article 22850. https://doi.org/10.1038/srep22850

@article{d3ebff5171394618b8f931ed08818d2a,

title = "Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells",

abstract = "Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment.",

author = "Rosanna Mezzapelle and Eltjona Rrapaj and Elena Gatti and Chiara Ceriotti and \{De Marchis\}, Francesco and Alessandro Preti and Spinelli, \{Antonello E.\} and Laura Perani and Massimo Venturini and Silvia Valtorta and Moresco, \{Rosa Maria\} and Lorenza Pecciarini and Claudio Doglioni and Michela Frenquelli and Luca Crippa and Camilla Recordati and Eugenio Scanziani and \{De Vries\}, Hilda and Anton Berns and Roberta Frapolli and Renzo Boldorini and Maurizio D'Incalci and Bianchi, \{Marco E.\} and Crippa, \{Massimo P.\}",

note = "Funding Information: We thank Mariacarla Panzeri at the ALEMBIC facility (S. Raffaele Hospital) for EM pictures. This work was supported by grants from A.I.R.C. (Associazione Nazionale per la Ricerca sul Cancro), number IG-14233, and Ministero della Salute, number RF-2009-1525236, to M.E.B.",

year = "2016",

month = mar,

day = "10",

doi = "10.1038/srep22850",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

Mezzapelle, R, Rrapaj, E, Gatti, E, Ceriotti, C, De Marchis, F, Preti, A, Spinelli, AE, Perani, L, Venturini, M, Valtorta, S, Moresco, RM, Pecciarini, L, Doglioni, C, Frenquelli, M, Crippa, L, Recordati, C, Scanziani, E, De Vries, H, Berns, A, Frapolli, R, Boldorini, R, D'Incalci, M, Bianchi, ME & Crippa, MP 2016, 'Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells', Scientific Reports, vol. 6, 22850. https://doi.org/10.1038/srep22850

TY - JOUR

T1 - Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells

AU - Mezzapelle, Rosanna

AU - Rrapaj, Eltjona

AU - Gatti, Elena

AU - Ceriotti, Chiara

AU - De Marchis, Francesco

AU - Preti, Alessandro

AU - Spinelli, Antonello E.

AU - Perani, Laura

AU - Venturini, Massimo

AU - Valtorta, Silvia

AU - Moresco, Rosa Maria

AU - Pecciarini, Lorenza

AU - Doglioni, Claudio

AU - Frenquelli, Michela

AU - Crippa, Luca

AU - Recordati, Camilla

AU - Scanziani, Eugenio

AU - De Vries, Hilda

AU - Berns, Anton

AU - Frapolli, Roberta

AU - Boldorini, Renzo

AU - D'Incalci, Maurizio

AU - Bianchi, Marco E.

AU - Crippa, Massimo P.

N1 - Funding Information: We thank Mariacarla Panzeri at the ALEMBIC facility (S. Raffaele Hospital) for EM pictures. This work was supported by grants from A.I.R.C. (Associazione Nazionale per la Ricerca sul Cancro), number IG-14233, and Ministero della Salute, number RF-2009-1525236, to M.E.B.

PY - 2016/3/10

Y1 - 2016/3/10

N2 - Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment.

AB - Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment.

UR - https://www.scopus.com/pages/publications/84960891457

U2 - 10.1038/srep22850

DO - 10.1038/srep22850

M3 - Article

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 22850

ER -

Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells

Abstract

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