Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

D VIGO; SIMONA VILLANI; M FAUSTINI; PA ACCORSI; G GALEATI; M SPINACI; E MUNARI; V RUSSO; A ASTI; UBALDO CONTE; MARIA LUISA TORRE

doi:10.1089/ten.2005.11.709

Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

D VIGO
, SIMONA VILLANI
, M FAUSTINI
, PA ACCORSI
, G GALEATI
, M SPINACI
, E MUNARI
, V RUSSO
, A ASTI
, UBALDO CONTE
, MARIA LUISA TORRE

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

Original language	English
Pages (from-to)	709-714
Number of pages	6
Journal	Tissue Engineering
Volume	11
Issue number	5/6
DOIs	https://doi.org/10.1089/ten.2005.11.709
Publication status	Published - 1 Jan 2005

Keywords

3D granulosa cells culture
capsules
in vitro maturation

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10.1089/ten.2005.11.709

http://hdl.handle.net/11579/144465

Cite this

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title = "Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane",

abstract = "Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.",

keywords = "3D granulosa cells culture, capsules, in vitro maturation, 3D granulosa cells culture, capsules, in vitro maturation",

author = "D VIGO and SIMONA VILLANI and M FAUSTINI and PA ACCORSI and G GALEATI and M SPINACI and E MUNARI and V RUSSO and A ASTI and UBALDO CONTE and TORRE, \{MARIA LUISA\}",

year = "2005",

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doi = "10.1089/ten.2005.11.709",

language = "English",

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TY - JOUR

T1 - Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

AU - VIGO, D

AU - VILLANI, SIMONA

AU - FAUSTINI, M

AU - ACCORSI, PA

AU - GALEATI, G

AU - SPINACI, M

AU - MUNARI, E

AU - RUSSO, V

AU - ASTI, A

AU - CONTE, UBALDO

AU - TORRE, MARIA LUISA

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

AB - Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

KW - 3D granulosa cells culture

KW - capsules

KW - in vitro maturation

KW - 3D granulosa cells culture

KW - capsules

KW - in vitro maturation

UR - https://iris.uniupo.it/handle/11579/144465

U2 - 10.1089/ten.2005.11.709

DO - 10.1089/ten.2005.11.709

M3 - Article

SN - 1076-3279

VL - 11

SP - 709

EP - 714

JO - Tissue Engineering

JF - Tissue Engineering

IS - 5/6

ER -

Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

Abstract

Keywords

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