Drug sensitivity tests after cell separation on density gradients

L. Silvestro; I. Viano; G. Compagnoni; M. L. Soffiantino; C. Vercellone; E. Genazzani

Drug sensitivity tests after cell separation on density gradients

L. Silvestro
, I. Viano
, G. Compagnoni
, M. L. Soffiantino
, C. Vercellone
, E. Genazzani

Research output: Contribution to journal › Article › peer-review

Abstract

Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5% discordance between the results for the fractions and the non-separated cells.

Original language	English
Pages (from-to)	119-123
Number of pages	5
Journal	Anticancer Research
Volume	8
Issue number	1
Publication status	Published - 1988
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Cite this

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title = "Drug sensitivity tests after cell separation on density gradients",

abstract = "Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5\% discordance between the results for the fractions and the non-separated cells.",

author = "L. Silvestro and I. Viano and G. Compagnoni and Soffiantino, \{M. L.\} and C. Vercellone and E. Genazzani",

year = "1988",

language = "English",

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journal = "Anticancer Research",

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publisher = "International Institute of Anticancer Research",

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TY - JOUR

T1 - Drug sensitivity tests after cell separation on density gradients

AU - Silvestro, L.

AU - Viano, I.

AU - Compagnoni, G.

AU - Soffiantino, M. L.

AU - Vercellone, C.

AU - Genazzani, E.

PY - 1988

Y1 - 1988

N2 - Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5% discordance between the results for the fractions and the non-separated cells.

AB - Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5% discordance between the results for the fractions and the non-separated cells.

UR - https://www.scopus.com/pages/publications/0023940747

M3 - Article

SN - 0250-7005

VL - 8

SP - 119

EP - 123

JO - Anticancer Research

JF - Anticancer Research

IS - 1

ER -

Drug sensitivity tests after cell separation on density gradients

Abstract

UN SDGs

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