DNA fragmentation is a feature of cystic fibrosis epithelial cells: A disease with inappropriate apoptosis?

Luigi Maiuri; Valeria Raia; Giulio De Marco; Salvatore Coletta; Giorgio De Ritis; Marco Londei; Salvatore Auricchio

doi:10.1016/S0014-5793(97)00347-5

DNA fragmentation is a feature of cystic fibrosis epithelial cells: A disease with inappropriate apoptosis?

Luigi Maiuri
, Valeria Raia
, Giulio De Marco
, Salvatore Coletta
, Giorgio De Ritis
, Marco Londei
, Salvatore Auricchio

Research output: Contribution to journal › Article › peer-review

Abstract

Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1-7) in controls P < 0.001) and median 37.5% (range: 23-79) crypt enterocytes show Ki67 antigen (P < 0.001). In CF airways 57% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	FEBS Letters
Volume	408
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(97)00347-5
Publication status	Published - 19 May 1997
Externally published	Yes

Keywords

Apoptosis
Cystic fibrosis
Cystic fibrosis transmembrane conductance regulator
DNA fragmentation
Programmed cell death

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10.1016/S0014-5793(97)00347-5

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@article{d6825ee8ed9049b49033068b060caafb,

title = "DNA fragmentation is a feature of cystic fibrosis epithelial cells: A disease with inappropriate apoptosis?",

abstract = "Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46\% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3\% (range: 1-7) in controls P < 0.001) and median 37.5\% (range: 23-79) crypt enterocytes show Ki67 antigen (P < 0.001). In CF airways 57\% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.",

keywords = "Apoptosis, Cystic fibrosis, Cystic fibrosis transmembrane conductance regulator, DNA fragmentation, Programmed cell death",

author = "Luigi Maiuri and Valeria Raia and \{De Marco\}, Giulio and Salvatore Coletta and \{De Ritis\}, Giorgio and Marco Londei and Salvatore Auricchio",

note = "Funding Information: This work was supported by grant 2902(10/06/1992) {"}Prevenzione, diagnosi e cura della Fibrosi Cistica{"} from Regione Campania, Italy. The authors thank Prof. S. Cucchiara for endoscopic procedures.",

year = "1997",

month = may,

day = "19",

doi = "10.1016/S0014-5793(97)00347-5",

language = "English",

volume = "408",

pages = "225--231",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

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TY - JOUR

T1 - DNA fragmentation is a feature of cystic fibrosis epithelial cells

T2 - A disease with inappropriate apoptosis?

AU - Maiuri, Luigi

AU - Raia, Valeria

AU - De Marco, Giulio

AU - Coletta, Salvatore

AU - De Ritis, Giorgio

AU - Londei, Marco

AU - Auricchio, Salvatore

N1 - Funding Information: This work was supported by grant 2902(10/06/1992) "Prevenzione, diagnosi e cura della Fibrosi Cistica" from Regione Campania, Italy. The authors thank Prof. S. Cucchiara for endoscopic procedures.

PY - 1997/5/19

Y1 - 1997/5/19

N2 - Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1-7) in controls P < 0.001) and median 37.5% (range: 23-79) crypt enterocytes show Ki67 antigen (P < 0.001). In CF airways 57% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.

AB - Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1-7) in controls P < 0.001) and median 37.5% (range: 23-79) crypt enterocytes show Ki67 antigen (P < 0.001). In CF airways 57% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.

KW - Apoptosis

KW - Cystic fibrosis

KW - Cystic fibrosis transmembrane conductance regulator

KW - DNA fragmentation

KW - Programmed cell death

UR - https://www.scopus.com/pages/publications/0030926711

U2 - 10.1016/S0014-5793(97)00347-5

DO - 10.1016/S0014-5793(97)00347-5

M3 - Article

SN - 0014-5793

VL - 408

SP - 225

EP - 231

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

DNA fragmentation is a feature of cystic fibrosis epithelial cells: A disease with inappropriate apoptosis?

Abstract

Keywords

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