DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA-CELLS BY HEXAMETHYLENEBISACETAMIDE INVOLVES SECRETION AND BINDING TO MEMBRANES OF A DIFFERENTIATION ENHANCING FACTOR

B SPARATORE; Mauro PATRONE; M. PASSALACQUA

doi:10.1016/0006-291X(91)91348-G

DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA-CELLS BY HEXAMETHYLENEBISACETAMIDE INVOLVES SECRETION AND BINDING TO MEMBRANES OF A DIFFERENTIATION ENHANCING FACTOR

B SPARATORE
, Mauro PATRONE
, M. PASSALACQUA

Department of Science and Technological Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

A protein factor previously shown to enhance terminal differentiation of transformed erythroid cells is synthesized by murine erythroleukemia cells and secreted in the early stages of differentiation induced by hexamethylenebisacetamide (HMBA). Secretion also occurs, constitutively, in the absence of inducer, from a murine erythroleukemia cell variant characterized by an accelerated response to HMBA. The protein factor binds to intact cells following addition of HMBA and enhances translocation of protein kinase C to the nuclear fraction. These results strongly support an important role for this natural protein factor in cell differentiation.

Original language	English
Pages (from-to)	153-160
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	179
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(91)91348-G
Publication status	Published - 1991

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10.1016/0006-291X(91)91348-G

http://hdl.handle.net/11579/3581

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@article{88e3daae20434bb69776bc8fd4714acc,

title = "DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA-CELLS BY HEXAMETHYLENEBISACETAMIDE INVOLVES SECRETION AND BINDING TO MEMBRANES OF A DIFFERENTIATION ENHANCING FACTOR",

abstract = "A protein factor previously shown to enhance terminal differentiation of transformed erythroid cells is synthesized by murine erythroleukemia cells and secreted in the early stages of differentiation induced by hexamethylenebisacetamide (HMBA). Secretion also occurs, constitutively, in the absence of inducer, from a murine erythroleukemia cell variant characterized by an accelerated response to HMBA. The protein factor binds to intact cells following addition of HMBA and enhances translocation of protein kinase C to the nuclear fraction. These results strongly support an important role for this natural protein factor in cell differentiation.",

author = "B SPARATORE and Mauro PATRONE and M. PASSALACQUA",

note = "Funding Information: ACKNOWLEDGMENTWSe: thank Dr. R.A. Rifkind for his skillful discussion and revision of the manuscript. This work was supported in part by grants from the Italian Minister0 dell'Universit\& e della Ricerca Scientifica e Tecnologica, from the National Research Council (C.N.R.) Target Project {"}Biotecnologie e Biostrumentazione{"} and {"}Ingegneria Genetica{"} and from the Associazione Italiana per la Ricerca sul Cancro.",

year = "1991",

doi = "10.1016/0006-291X(91)91348-G",

language = "English",

volume = "179",

pages = "153--160",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA-CELLS BY HEXAMETHYLENEBISACETAMIDE INVOLVES SECRETION AND BINDING TO MEMBRANES OF A DIFFERENTIATION ENHANCING FACTOR

AU - SPARATORE, B

AU - PATRONE, Mauro

AU - PASSALACQUA, M.

N1 - Funding Information: ACKNOWLEDGMENTWSe: thank Dr. R.A. Rifkind for his skillful discussion and revision of the manuscript. This work was supported in part by grants from the Italian Minister0 dell'Universit& e della Ricerca Scientifica e Tecnologica, from the National Research Council (C.N.R.) Target Project "Biotecnologie e Biostrumentazione" and "Ingegneria Genetica" and from the Associazione Italiana per la Ricerca sul Cancro.

PY - 1991

Y1 - 1991

N2 - A protein factor previously shown to enhance terminal differentiation of transformed erythroid cells is synthesized by murine erythroleukemia cells and secreted in the early stages of differentiation induced by hexamethylenebisacetamide (HMBA). Secretion also occurs, constitutively, in the absence of inducer, from a murine erythroleukemia cell variant characterized by an accelerated response to HMBA. The protein factor binds to intact cells following addition of HMBA and enhances translocation of protein kinase C to the nuclear fraction. These results strongly support an important role for this natural protein factor in cell differentiation.

AB - A protein factor previously shown to enhance terminal differentiation of transformed erythroid cells is synthesized by murine erythroleukemia cells and secreted in the early stages of differentiation induced by hexamethylenebisacetamide (HMBA). Secretion also occurs, constitutively, in the absence of inducer, from a murine erythroleukemia cell variant characterized by an accelerated response to HMBA. The protein factor binds to intact cells following addition of HMBA and enhances translocation of protein kinase C to the nuclear fraction. These results strongly support an important role for this natural protein factor in cell differentiation.

UR - https://iris.uniupo.it/handle/11579/3581

U2 - 10.1016/0006-291X(91)91348-G

DO - 10.1016/0006-291X(91)91348-G

M3 - Article

SN - 0006-291X

VL - 179

SP - 153

EP - 160

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA-CELLS BY HEXAMETHYLENEBISACETAMIDE INVOLVES SECRETION AND BINDING TO MEMBRANES OF A DIFFERENTIATION ENHANCING FACTOR

Abstract

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