Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

C. Robino; A. Giolitti; S. Gino; C. Torre

doi:10.1007/s00414-006-0115-9

Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

C. Robino
, A. Giolitti
, S. Gino
, C. Torre

Research output: Contribution to journal › Article › peer-review

Abstract

Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101-DXS7424 and DXS6789-DXS6801-DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.

Original language	English
Pages (from-to)	315-318
Number of pages	4
Journal	International Journal of Legal Medicine
Volume	120
Issue number	5
DOIs	https://doi.org/10.1007/s00414-006-0115-9
Publication status	Published - Sept 2006
Externally published	Yes

Keywords

DXS6789
Italy
Multiplex PCR
Short tandem repeats
X chromosome

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10.1007/s00414-006-0115-9

Cite this

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title = "Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample",

abstract = "Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101-DXS7424 and DXS6789-DXS6801-DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.",

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T1 - Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

AU - Robino, C.

AU - Giolitti, A.

AU - Gino, S.

AU - Torre, C.

PY - 2006/9

Y1 - 2006/9

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AB - Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101-DXS7424 and DXS6789-DXS6801-DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.

KW - DXS6789

KW - Italy

KW - Multiplex PCR

KW - Short tandem repeats

KW - X chromosome

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DO - 10.1007/s00414-006-0115-9

M3 - Article

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EP - 318

JO - International Journal of Legal Medicine

JF - International Journal of Legal Medicine

IS - 5

ER -

Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

Abstract

Keywords

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