Determination of H2S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata

Alberto Boffi; Menico Rizzi; Fabrizio Monacelli; Paolo Ascenzi

doi:10.1016/S0304-4165(00)00123-9

Determination of H₂S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata

Alberto Boffi
, Menico Rizzi
, Fabrizio Monacelli
, Paolo Ascenzi

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A new, simple and fast spectrophotometric method for the determination of the H₂S concentration is reported. This method, based on the 1:1 reaction between H₂S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H₂S dissolved in a given solution even at concentrations as low as 1 x 10^-6 M. Note that L. pectinata HbI is considered the physiological receptor of H₂S. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	206-208
Number of pages	3
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	1523
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-4165(00)00123-9
Publication status	Published - 18 Oct 2000

Keywords

HS binding to Lucina pectinata hemoglobin I
HS solubility
Lucina pectinata hemoglobin I

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10.1016/S0304-4165(00)00123-9

Cite this

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title = "Determination of H2S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata",

abstract = "A new, simple and fast spectrophotometric method for the determination of the H2S concentration is reported. This method, based on the 1:1 reaction between H2S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H2S dissolved in a given solution even at concentrations as low as 1 x 10-6 M. Note that L. pectinata HbI is considered the physiological receptor of H2S. (C) 2000 Elsevier Science B.V.",

keywords = "HS binding to Lucina pectinata hemoglobin I, HS solubility, Lucina pectinata hemoglobin I",

author = "Alberto Boffi and Menico Rizzi and Fabrizio Monacelli and Paolo Ascenzi",

note = "Funding Information: The authors wish to thank Prof. M. Bolognesi and Prof. J.B. Wittenberg for helpful discussions. This work was supported by grants from the Ministry of University, Scientific Research and Technology of Italy (target oriented project {\textquoteleft}Biocatalisi e Bioconversioni{\textquoteright}) and from the National Research Council of Italy (target oriented project {\textquoteleft}Biotecnologie{\textquoteright}).",

year = "2000",

month = oct,

day = "18",

doi = "10.1016/S0304-4165(00)00123-9",

language = "English",

volume = "1523",

pages = "206--208",

journal = "Biochimica et Biophysica Acta - General Subjects",

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TY - JOUR

T1 - Determination of H2S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata

AU - Boffi, Alberto

AU - Rizzi, Menico

AU - Monacelli, Fabrizio

AU - Ascenzi, Paolo

N1 - Funding Information: The authors wish to thank Prof. M. Bolognesi and Prof. J.B. Wittenberg for helpful discussions. This work was supported by grants from the Ministry of University, Scientific Research and Technology of Italy (target oriented project ‘Biocatalisi e Bioconversioni’) and from the National Research Council of Italy (target oriented project ‘Biotecnologie’).

PY - 2000/10/18

Y1 - 2000/10/18

N2 - A new, simple and fast spectrophotometric method for the determination of the H2S concentration is reported. This method, based on the 1:1 reaction between H2S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H2S dissolved in a given solution even at concentrations as low as 1 x 10-6 M. Note that L. pectinata HbI is considered the physiological receptor of H2S. (C) 2000 Elsevier Science B.V.

AB - A new, simple and fast spectrophotometric method for the determination of the H2S concentration is reported. This method, based on the 1:1 reaction between H2S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H2S dissolved in a given solution even at concentrations as low as 1 x 10-6 M. Note that L. pectinata HbI is considered the physiological receptor of H2S. (C) 2000 Elsevier Science B.V.

KW - HS binding to Lucina pectinata hemoglobin I

KW - HS solubility

KW - Lucina pectinata hemoglobin I

UR - https://www.scopus.com/pages/publications/0034684222

U2 - 10.1016/S0304-4165(00)00123-9

DO - 10.1016/S0304-4165(00)00123-9

M3 - Article

SN - 0304-4165

VL - 1523

SP - 206

EP - 208

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

IS - 2-3

ER -

Determination of H₂S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata

Abstract

Keywords

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