TY - JOUR
T1 - Detection of cytochrome P4503A (CYP3A) in human hepatic stellate cells
AU - Parola, Maurizio
AU - Robino, Gaia
AU - Bordone, Roberta
AU - Leonarduzzi, Gabriella
AU - Casini, Alessandro
AU - Pinzani, Massimo
AU - Neve, Etienne
AU - Bellomo, Giorgio
AU - Dianzani, Mario U.
AU - Ingelman-Sundberg, Magnus
AU - Albano, Emanuele
N1 - Funding Information:
Financial support for research described in this paper was provided by Italian Ministry for University and Scienti®c and Technologic Research (National Projects on ``Cirrosi Epatica ed Epatiti Vi-rali'' and ``Patologia da Radicali Liberi e degli Equilibri Redox''), by Italian National Research Council (ACRO Project), by Italian Association for Cancer Research (AIRC), by Italian Liver Foundation, by Swedish Alcohol Research Fund and Swedish Medical Research Council.
PY - 1997/9/18
Y1 - 1997/9/18
N2 - In this study we have investigated the occurrence of cytochrome P450 isoforms and of related cytochrome P450 reductase in human hepatic stellate cells (hHSC), a type of cell having relevant roles in physiopathological conditions of the liver. By performing immunoblotting of hHSC microsomes and immunofluorescence analysis associated to confocal laser microscopy we detected only P450 enzymes belonging to the cytochrome P450 3A subfamily (CYP3A) as well as cytochrome P450 reductase. The presence of CYP3A was further indicated by detection of testosterone 6β-hydroxylase activity in hHSC microsomes. Other important human P450 forms were either undetectable (CYP1A2, CYP2E1, CYP2C8/9/19 and CYP4A) or bearly detectable (CYP1A1) in hHSC. This is the first study showing existence of active cytochrome P450 isoforms in human HSC.
AB - In this study we have investigated the occurrence of cytochrome P450 isoforms and of related cytochrome P450 reductase in human hepatic stellate cells (hHSC), a type of cell having relevant roles in physiopathological conditions of the liver. By performing immunoblotting of hHSC microsomes and immunofluorescence analysis associated to confocal laser microscopy we detected only P450 enzymes belonging to the cytochrome P450 3A subfamily (CYP3A) as well as cytochrome P450 reductase. The presence of CYP3A was further indicated by detection of testosterone 6β-hydroxylase activity in hHSC microsomes. Other important human P450 forms were either undetectable (CYP1A2, CYP2E1, CYP2C8/9/19 and CYP4A) or bearly detectable (CYP1A1) in hHSC. This is the first study showing existence of active cytochrome P450 isoforms in human HSC.
UR - https://www.scopus.com/pages/publications/0031577580
U2 - 10.1006/bbrc.1997.7307
DO - 10.1006/bbrc.1997.7307
M3 - Article
SN - 0006-291X
VL - 238
SP - 420
EP - 424
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -