Core-shell nanospheres for oligonucleotide deliver. V: Adsorption/release behavior of 'stealth' nanospheres

Luisa Tondelli; Marco Ballestri; Laura Magnani; Daniela Vivarelli; Adamo Fini; Aurora Cerasi; Laura Chiarantini; Katia Sparnacci; Michele Laus

doi:10.1163/156856203322553446

Core-shell nanospheres for oligonucleotide deliver. V: Adsorption/release behavior of 'stealth' nanospheres

Luisa Tondelli
, Marco Ballestri
, Laura Magnani
, Daniela Vivarelli
, Adamo Fini
, Aurora Cerasi
, Laura Chiarantini
, Katia Sparnacci
, Michele Laus

Department of Science and Technological Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

The adsorption/release behavior of oligodeoxynucleotides (ODNs) on new PEGylated core-shell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.

Original language	English
Pages (from-to)	1209-1227
Number of pages	19
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	14
Issue number	11
DOIs	https://doi.org/10.1163/156856203322553446
Publication status	Published - 2003

Keywords

Adsorption
Antisense oligonucleotides
Core-shell nanospheres
Cytotoxicity
PEG
Release
Stealth

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10.1163/156856203322553446

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title = "Core-shell nanospheres for oligonucleotide deliver. V: Adsorption/release behavior of 'stealth' nanospheres",

abstract = "The adsorption/release behavior of oligodeoxynucleotides (ODNs) on new PEGylated core-shell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.",

keywords = "Adsorption, Antisense oligonucleotides, Core-shell nanospheres, Cytotoxicity, PEG, Release, Stealth",

author = "Luisa Tondelli and Marco Ballestri and Laura Magnani and Daniela Vivarelli and Adamo Fini and Aurora Cerasi and Laura Chiarantini and Katia Sparnacci and Michele Laus",

year = "2003",

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language = "English",

volume = "14",

pages = "1209--1227",

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TY - JOUR

T1 - Core-shell nanospheres for oligonucleotide deliver. V

T2 - Adsorption/release behavior of 'stealth' nanospheres

AU - Tondelli, Luisa

AU - Ballestri, Marco

AU - Magnani, Laura

AU - Vivarelli, Daniela

AU - Fini, Adamo

AU - Cerasi, Aurora

AU - Chiarantini, Laura

AU - Sparnacci, Katia

AU - Laus, Michele

PY - 2003

Y1 - 2003

N2 - The adsorption/release behavior of oligodeoxynucleotides (ODNs) on new PEGylated core-shell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.

AB - The adsorption/release behavior of oligodeoxynucleotides (ODNs) on new PEGylated core-shell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.

KW - Adsorption

KW - Antisense oligonucleotides

KW - Core-shell nanospheres

KW - Cytotoxicity

KW - PEG

KW - Release

KW - Stealth

UR - https://www.scopus.com/pages/publications/0344035325

U2 - 10.1163/156856203322553446

DO - 10.1163/156856203322553446

M3 - Article

SN - 0920-5063

VL - 14

SP - 1209

EP - 1227

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

IS - 11

ER -

Core-shell nanospheres for oligonucleotide deliver. V: Adsorption/release behavior of 'stealth' nanospheres

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Keywords

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