Construction of a genetic map telomeric to HLA-A by microsatellite analysis

C. Camaschella; A. Roetto; G. De Sandre; A. Piperno; A. Totaro; I. Dianzani; P. Gasparini

doi:10.1006/mcpr.1993.1060

Construction of a genetic map telomeric to HLA-A by microsatellite analysis

C. Camaschella
, A. Roetto
, G. De Sandre
, A. Piperno
, A. Totaro
, I. Dianzani
, P. Gasparini

Research output: Contribution to journal › Article › peer-review

Abstract

Allele and genotype frequencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0·65 to 0·78. Two point and three point linkage anlaysis among these markers and HLA-A serological types-available for all the studied samples-allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage analysis of genetic disorders mapping on this region of 6p.

Original language	English
Pages (from-to)	411-414
Number of pages	4
Journal	Molecular and Cellular Probes
Volume	7
Issue number	5
DOIs	https://doi.org/10.1006/mcpr.1993.1060
Publication status	Published - Oct 1993
Externally published	Yes

Keywords

Genetic map, linkage analysis, chromosome 6, microsatellites

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10.1006/mcpr.1993.1060

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title = "Construction of a genetic map telomeric to HLA-A by microsatellite analysis",

abstract = "Allele and genotype frequencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0·65 to 0·78. Two point and three point linkage anlaysis among these markers and HLA-A serological types-available for all the studied samples-allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage analysis of genetic disorders mapping on this region of 6p.",

keywords = "Genetic map, linkage analysis, chromosome 6, microsatellites",

author = "C. Camaschella and A. Roetto and \{De Sandre\}, G. and A. Piperno and A. Totaro and I. Dianzani and P. Gasparini",

year = "1993",

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doi = "10.1006/mcpr.1993.1060",

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pages = "411--414",

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TY - JOUR

T1 - Construction of a genetic map telomeric to HLA-A by microsatellite analysis

AU - Camaschella, C.

AU - Roetto, A.

AU - De Sandre, G.

AU - Piperno, A.

AU - Totaro, A.

AU - Dianzani, I.

AU - Gasparini, P.

PY - 1993/10

Y1 - 1993/10

N2 - Allele and genotype frequencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0·65 to 0·78. Two point and three point linkage anlaysis among these markers and HLA-A serological types-available for all the studied samples-allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage analysis of genetic disorders mapping on this region of 6p.

AB - Allele and genotype frequencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0·65 to 0·78. Two point and three point linkage anlaysis among these markers and HLA-A serological types-available for all the studied samples-allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage analysis of genetic disorders mapping on this region of 6p.

KW - Genetic map, linkage analysis, chromosome 6, microsatellites

UR - https://www.scopus.com/pages/publications/0027361646

U2 - 10.1006/mcpr.1993.1060

DO - 10.1006/mcpr.1993.1060

M3 - Article

SN - 0890-8508

VL - 7

SP - 411

EP - 414

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

IS - 5

ER -

Construction of a genetic map telomeric to HLA-A by microsatellite analysis

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Keywords

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