Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates

Elisa Caristo; Andrea Parola; Anna Rapa; Daniela Vivenza; Barbara Raselli; Elena Dondi; Renzo Boldorini; Giuseppina Oderda

doi:10.1111/j.1523-5378.2008.00642.x

Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates

Elisa Caristo
, Andrea Parola
, Anna Rapa
, Daniela Vivenza
, Barbara Raselli
, Elena Dondi
, Renzo Boldorini
, Giuseppina Oderda

Department of Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To assess validity of culture on four-sector agar plates and fluorescent in-situ hybridization (FISH) test, and clarithromycin resistance rate in Helicobacter pylori strains isolated from children in the last 10 years. Methods: In the last 5 years, gastric biopsy specimens from antrum and fundus were taken from 89 consecutive children (median age 9 years) with H. pylori gastritis and from 21 controls. Culture was performed on 176 gastric biopsies (89 from antrum, 87 from fundus) on four-sector agar plates, and FISH test with DNA ProbeMix^®. After its validity was evaluated, FISH test was applied on additional 119 biopsies from 68 children (68 from the antrum, 51 from the fundus) stored in the Pathology archive in the previous 5 years. Results: Culture was positive in 157 of 176 biopsies (sensitivity: 89.2%, 95% confidence interval (CI) 85-94). In 33 of 89 children (37%) resistant strains were found in one or both gastric sites. FISH test was positive in 148 of 176 biopsies from infected children (sensitivity 84.1%, 95%CI 79-89) and in none of 42 biopsies from controls (specificity 100%). When applied on archive biopsies, FISH test was positive in 96 of 119 (80.7%, 95%CI 74-88). Total children harboring resistant strains in the last 10 years, as assessed by FISH test, were 66 of 157 (42%). Mixed infection with both sensitive and resistant strains were found in 40 children (25%) and in 12 of them resistant strains were in the fundus only. Conclusions: Culture on four-sector agar plates and FISH test had a high sensitivity and specificity and showed co-presence of sensitive and resistant strains. In one-third of children with mixed infection, the resistant strains were in the fundus only. Clarithromycin resistance should be assessed in biopsies both from the antrum and the fundus, utilizing antral biopsies only can underestimate its prevalence.

Original language	English
Pages (from-to)	557-563
Number of pages	7
Journal	Helicobacter
Volume	13
Issue number	6
DOIs	https://doi.org/10.1111/j.1523-5378.2008.00642.x
Publication status	Published - Dec 2008

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antibiotic susceptibility
Children
Clarithromycin
FISH test
Helicobacter pylori

Access to Document

10.1111/j.1523-5378.2008.00642.x

Cite this

Caristo, E., Parola, A., Rapa, A., Vivenza, D., Raselli, B., Dondi, E., Boldorini, R., & Oderda, G. (2008). Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates. Helicobacter, 13(6), 557-563. https://doi.org/10.1111/j.1523-5378.2008.00642.x

@article{df693188d6d84fb1aecf19e15167301d,

title = "Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates",

abstract = "Aim: To assess validity of culture on four-sector agar plates and fluorescent in-situ hybridization (FISH) test, and clarithromycin resistance rate in Helicobacter pylori strains isolated from children in the last 10 years. Methods: In the last 5 years, gastric biopsy specimens from antrum and fundus were taken from 89 consecutive children (median age 9 years) with H. pylori gastritis and from 21 controls. Culture was performed on 176 gastric biopsies (89 from antrum, 87 from fundus) on four-sector agar plates, and FISH test with DNA ProbeMix{\textregistered}. After its validity was evaluated, FISH test was applied on additional 119 biopsies from 68 children (68 from the antrum, 51 from the fundus) stored in the Pathology archive in the previous 5 years. Results: Culture was positive in 157 of 176 biopsies (sensitivity: 89.2\%, 95\% confidence interval (CI) 85-94). In 33 of 89 children (37\%) resistant strains were found in one or both gastric sites. FISH test was positive in 148 of 176 biopsies from infected children (sensitivity 84.1\%, 95\%CI 79-89) and in none of 42 biopsies from controls (specificity 100\%). When applied on archive biopsies, FISH test was positive in 96 of 119 (80.7\%, 95\%CI 74-88). Total children harboring resistant strains in the last 10 years, as assessed by FISH test, were 66 of 157 (42\%). Mixed infection with both sensitive and resistant strains were found in 40 children (25\%) and in 12 of them resistant strains were in the fundus only. Conclusions: Culture on four-sector agar plates and FISH test had a high sensitivity and specificity and showed co-presence of sensitive and resistant strains. In one-third of children with mixed infection, the resistant strains were in the fundus only. Clarithromycin resistance should be assessed in biopsies both from the antrum and the fundus, utilizing antral biopsies only can underestimate its prevalence.",

keywords = "Antibiotic susceptibility, Children, Clarithromycin, FISH test, Helicobacter pylori",

author = "Elisa Caristo and Andrea Parola and Anna Rapa and Daniela Vivenza and Barbara Raselli and Elena Dondi and Renzo Boldorini and Giuseppina Oderda",

note = "Funding Information: 'This work was prepared through collaborative participation in the Collaborative Technology Alliance for Cornn\textasciitilde{}unicationsa nd Networks sponsored by the U.S. Army Research Laboratory under Cooperative Agreement DAAD19-01-2-0011. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation thereon.",

year = "2008",

month = dec,

doi = "10.1111/j.1523-5378.2008.00642.x",

language = "English",

volume = "13",

pages = "557--563",

journal = "Helicobacter",

issn = "1083-4389",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "6",

}

Caristo, E, Parola, A, Rapa, A, Vivenza, D, Raselli, B, Dondi, E, Boldorini, R & Oderda, G 2008, 'Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates', Helicobacter, vol. 13, no. 6, pp. 557-563. https://doi.org/10.1111/j.1523-5378.2008.00642.x

Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates. / Caristo, Elisa; Parola, Andrea; Rapa, Anna et al.
In: Helicobacter, Vol. 13, No. 6, 12.2008, p. 557-563.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates

AU - Caristo, Elisa

AU - Parola, Andrea

AU - Rapa, Anna

AU - Vivenza, Daniela

AU - Raselli, Barbara

AU - Dondi, Elena

AU - Boldorini, Renzo

AU - Oderda, Giuseppina

N1 - Funding Information: 'This work was prepared through collaborative participation in the Collaborative Technology Alliance for Cornn~unicationsa nd Networks sponsored by the U.S. Army Research Laboratory under Cooperative Agreement DAAD19-01-2-0011. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation thereon.

PY - 2008/12

Y1 - 2008/12

N2 - Aim: To assess validity of culture on four-sector agar plates and fluorescent in-situ hybridization (FISH) test, and clarithromycin resistance rate in Helicobacter pylori strains isolated from children in the last 10 years. Methods: In the last 5 years, gastric biopsy specimens from antrum and fundus were taken from 89 consecutive children (median age 9 years) with H. pylori gastritis and from 21 controls. Culture was performed on 176 gastric biopsies (89 from antrum, 87 from fundus) on four-sector agar plates, and FISH test with DNA ProbeMix®. After its validity was evaluated, FISH test was applied on additional 119 biopsies from 68 children (68 from the antrum, 51 from the fundus) stored in the Pathology archive in the previous 5 years. Results: Culture was positive in 157 of 176 biopsies (sensitivity: 89.2%, 95% confidence interval (CI) 85-94). In 33 of 89 children (37%) resistant strains were found in one or both gastric sites. FISH test was positive in 148 of 176 biopsies from infected children (sensitivity 84.1%, 95%CI 79-89) and in none of 42 biopsies from controls (specificity 100%). When applied on archive biopsies, FISH test was positive in 96 of 119 (80.7%, 95%CI 74-88). Total children harboring resistant strains in the last 10 years, as assessed by FISH test, were 66 of 157 (42%). Mixed infection with both sensitive and resistant strains were found in 40 children (25%) and in 12 of them resistant strains were in the fundus only. Conclusions: Culture on four-sector agar plates and FISH test had a high sensitivity and specificity and showed co-presence of sensitive and resistant strains. In one-third of children with mixed infection, the resistant strains were in the fundus only. Clarithromycin resistance should be assessed in biopsies both from the antrum and the fundus, utilizing antral biopsies only can underestimate its prevalence.

AB - Aim: To assess validity of culture on four-sector agar plates and fluorescent in-situ hybridization (FISH) test, and clarithromycin resistance rate in Helicobacter pylori strains isolated from children in the last 10 years. Methods: In the last 5 years, gastric biopsy specimens from antrum and fundus were taken from 89 consecutive children (median age 9 years) with H. pylori gastritis and from 21 controls. Culture was performed on 176 gastric biopsies (89 from antrum, 87 from fundus) on four-sector agar plates, and FISH test with DNA ProbeMix®. After its validity was evaluated, FISH test was applied on additional 119 biopsies from 68 children (68 from the antrum, 51 from the fundus) stored in the Pathology archive in the previous 5 years. Results: Culture was positive in 157 of 176 biopsies (sensitivity: 89.2%, 95% confidence interval (CI) 85-94). In 33 of 89 children (37%) resistant strains were found in one or both gastric sites. FISH test was positive in 148 of 176 biopsies from infected children (sensitivity 84.1%, 95%CI 79-89) and in none of 42 biopsies from controls (specificity 100%). When applied on archive biopsies, FISH test was positive in 96 of 119 (80.7%, 95%CI 74-88). Total children harboring resistant strains in the last 10 years, as assessed by FISH test, were 66 of 157 (42%). Mixed infection with both sensitive and resistant strains were found in 40 children (25%) and in 12 of them resistant strains were in the fundus only. Conclusions: Culture on four-sector agar plates and FISH test had a high sensitivity and specificity and showed co-presence of sensitive and resistant strains. In one-third of children with mixed infection, the resistant strains were in the fundus only. Clarithromycin resistance should be assessed in biopsies both from the antrum and the fundus, utilizing antral biopsies only can underestimate its prevalence.

KW - Antibiotic susceptibility

KW - Children

KW - Clarithromycin

KW - FISH test

KW - Helicobacter pylori

UR - https://www.scopus.com/pages/publications/55949093741

U2 - 10.1111/j.1523-5378.2008.00642.x

DO - 10.1111/j.1523-5378.2008.00642.x

M3 - Article

SN - 1083-4389

VL - 13

SP - 557

EP - 563

JO - Helicobacter

JF - Helicobacter

IS - 6

ER -

Clarithromycin resistance of Helicobacter pylori strains isolated from children' gastric antrum and fundus as assessed by fluorescent in-situ hybridization and culture on four-sector agar plates

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this