Chitin degradation by Synechococcus WH7803

Giovanna Capovilla; Kurt G. Castro; Silvio Collani; Sean M. Kearney; David M. Kehoe; Sallie W. Chisholm

doi:10.1038/s41598-023-47332-0

Chitin degradation by Synechococcus WH7803

Giovanna Capovilla
, Kurt G. Castro
, Silvio Collani
, Sean M. Kearney
, David M. Kehoe
, Sallie W. Chisholm

Research output: Contribution to journal › Article › peer-review

Abstract

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

Original language	English
Article number	19944
Journal	Scientific Reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41598-023-47332-0
Publication status	Published - Dec 2023
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

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10.1038/s41598-023-47332-0

Cite this

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title = "Chitin degradation by Synechococcus WH7803",

abstract = "Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.",

author = "Giovanna Capovilla and Castro, \{Kurt G.\} and Silvio Collani and Kearney, \{Sean M.\} and Kehoe, \{David M.\} and Chisholm, \{Sallie W.\}",

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doi = "10.1038/s41598-023-47332-0",

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AU - Capovilla, Giovanna

AU - Castro, Kurt G.

AU - Collani, Silvio

AU - Kearney, Sean M.

AU - Kehoe, David M.

AU - Chisholm, Sallie W.

PY - 2023/12

Y1 - 2023/12

N2 - Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

AB - Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

UR - https://www.scopus.com/pages/publications/85176434547

U2 - 10.1038/s41598-023-47332-0

DO - 10.1038/s41598-023-47332-0

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VL - 13

JO - Scientific Reports

JF - Scientific Reports

IS - 1

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ER -

Chitin degradation by Synechococcus WH7803

Abstract

UN SDGs

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