Chitin degradation by Synechococcus WH7803

Giovanna Capovilla; Kurt G. Castro; SILVIO COLLANI; Sean M. Kearney; David M. Kehoe; Sallie W. Chisholm

doi:10.1038/s41598-023-47332-0

Chitin degradation by Synechococcus WH7803

Giovanna Capovilla, Kurt G. Castro, SILVIO COLLANI, Sean M. Kearney, David M. Kehoe, Sallie W. Chisholm

Department of Sustainable Development and Ecological Transition

Research output: Contribution to journal › Article › peer-review

Abstract

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

Original language	English
Journal	Scientific Reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41598-023-47332-0
Publication status	Published - 2023

Keywords

CRISPR
Synechococcus
chitin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41598-023-47332-0

https://hdl.handle.net/11579/194706

Cite this

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title = "Chitin degradation by Synechococcus WH7803",

abstract = "Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.",

keywords = "CRISPR, Synechococcus, chitin, CRISPR, Synechococcus, chitin",

author = "Giovanna Capovilla and Castro, {Kurt G.} and SILVIO COLLANI and Kearney, {Sean M.} and Kehoe, {David M.} and Chisholm, {Sallie W.}",

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doi = "10.1038/s41598-023-47332-0",

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journal = "Scientific Reports",

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AU - Capovilla, Giovanna

AU - Castro, Kurt G.

AU - COLLANI, SILVIO

AU - Kearney, Sean M.

AU - Kehoe, David M.

AU - Chisholm, Sallie W.

PY - 2023

Y1 - 2023

N2 - Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

AB - Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

KW - CRISPR

KW - Synechococcus

KW - chitin

KW - CRISPR

KW - Synechococcus

KW - chitin

UR - https://iris.uniupo.it/handle/11579/194706

U2 - 10.1038/s41598-023-47332-0

DO - 10.1038/s41598-023-47332-0

M3 - Article

SN - 2045-2322

VL - 13

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -

Chitin degradation by Synechococcus WH7803

Abstract

Keywords

UN SDGs

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