Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

A. Gallina; F. Rossi; G. Milanesi; R. Rossi; A. Montecucco; G. Ciarrocchi

doi:10.1042/bj3120593

Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

A. Gallina
, F. Rossi
, G. Milanesi
, R. Rossi
, A. Montecucco
, G. Ciarrocchi

Research output: Contribution to journal › Article › peer-review

Abstract

The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 10⁸ Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein

Original language	English
Pages (from-to)	593-597
Number of pages	5
Journal	Biochemical Journal
Volume	312
Issue number	2
DOIs	https://doi.org/10.1042/bj3120593
Publication status	Published - 1995
Externally published	Yes

Access to Document

10.1042/bj3120593

Cite this

@article{d994d803ef7c48b89b40a77ce6881994,

title = "Characterization of human DNA ligase I expressed in a baculovirus-insect cell system",

abstract = "The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 108 Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein",

author = "A. Gallina and F. Rossi and G. Milanesi and R. Rossi and A. Montecucco and G. Ciarrocchi",

year = "1995",

doi = "10.1042/bj3120593",

language = "English",

volume = "312",

pages = "593--597",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd",

number = "2",

}

TY - JOUR

T1 - Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

AU - Gallina, A.

AU - Rossi, F.

AU - Milanesi, G.

AU - Rossi, R.

AU - Montecucco, A.

AU - Ciarrocchi, G.

PY - 1995

Y1 - 1995

N2 - The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 108 Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein

AB - The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 108 Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein

UR - https://www.scopus.com/pages/publications/0029609364

U2 - 10.1042/bj3120593

DO - 10.1042/bj3120593

M3 - Article

SN - 0264-6021

VL - 312

SP - 593

EP - 597

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -

Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

Abstract

Access to Document

Other files and links

Fingerprint

Cite this