Cellulomonas fimi secretomes: In vivo and in silico approaches for the lignocellulose bioconversion

Stefano Spertino, Lara Boatti, Sara Icardi, Marcello Manfredi, Chiara Cattaneo, Emilio Marengo, Maria Cavaletto

Research output: Contribution to journalArticlepeer-review

Abstract

Lignocellulose degradation is a challenging step for value added products and biofuels production. Cellulomonas fimi secretes complex mixtures of carbohydrate active enzymes (CAZymes) which synergistically degrade cellulose and hemicelluloses. Their characterization may provide new insights for enzymatic cocktails implementation. Bioinformatic analysis highlighted 1127 secreted proteins, constituting the in silico secretome, graphically represented in a 2DE map. According to Blast2GO functional annotation, many of these are involved in carbohydrates metabolism. In vivo secretomes were obtained, growing C. fimi on glucose, CMC or wheat straw for 24 h. Zymography revealed degradative activity on carbohydrates and proteomic analysis identified some CAZymes, only in secretomes obtained with CMC and wheat straw. An interaction between cellobiohydrolases is proposed as a strategy adopted by soluble multimodular cellulases. Such approach can be crucial for a better characterization and industrial exploitation of the synergism among C. fimi enzymes.

Original languageEnglish
Pages (from-to)21-29
Number of pages9
JournalJournal of Biotechnology
Volume270
DOIs
Publication statusPublished - 20 Mar 2018

Keywords

  • Carbohydrate active enzymes
  • Cellulomonas fimi
  • In silico 2DE
  • Lignocellulose bioconversion
  • Optimal secretion médium
  • Secretome

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