Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

Gian Carlo Avanzi; Alessandra Cessano; Maria Felice Brizzi; Steven C. Clark; Luigi Pegoraro; Lina Matera

doi:10.1016/0024-3205(89)90247-6

Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

Gian Carlo Avanzi
, Alessandra Cessano
, Maria Felice Brizzi
, Steven C. Clark
, Luigi Pegoraro
, Lina Matera

Research output: Contribution to journal › Article › peer-review

Abstract

As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.

Original language	English
Pages (from-to)	2621-2626
Number of pages	6
Journal	Life Sciences
Volume	45
Issue number	26
DOIs	https://doi.org/10.1016/0024-3205(89)90247-6
Publication status	Published - 1989
Externally published	Yes

Access to Document

10.1016/0024-3205(89)90247-6

Cite this

@article{3e3daebdefe749b8a0e5fddfed47c703,

title = "Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture",

abstract = "As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.",

author = "Avanzi, \{Gian Carlo\} and Alessandra Cessano and Brizzi, \{Maria Felice\} and Clark, \{Steven C.\} and Luigi Pegoraro and Lina Matera",

note = "Funding Information: This work was supported by CNR, Progetto and by Comitato Gigi Ghirotti.",

year = "1989",

doi = "10.1016/0024-3205(89)90247-6",

language = "English",

volume = "45",

pages = "2621--2626",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "26",

}

TY - JOUR

T1 - Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

AU - Avanzi, Gian Carlo

AU - Cessano, Alessandra

AU - Brizzi, Maria Felice

AU - Clark, Steven C.

AU - Pegoraro, Luigi

AU - Matera, Lina

N1 - Funding Information: This work was supported by CNR, Progetto and by Comitato Gigi Ghirotti.

PY - 1989

Y1 - 1989

N2 - As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.

AB - As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.

UR - https://www.scopus.com/pages/publications/0024791942

U2 - 10.1016/0024-3205(89)90247-6

DO - 10.1016/0024-3205(89)90247-6

M3 - Article

SN - 0024-3205

VL - 45

SP - 2621

EP - 2626

JO - Life Sciences

JF - Life Sciences

IS - 26

ER -

Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

Abstract

Access to Document

Other files and links

Fingerprint

Cite this