Bioencapsulation of Oocytes and Granulosa Cells

Massimo Faustini; Giulio Curone; MARIA LUISA TORRE; Daniele Vigo

doi:10.1007/978-1-4939-8600-2_9

Bioencapsulation of Oocytes and Granulosa Cells

Massimo Faustini, Giulio Curone, MARIA LUISA TORRE, Daniele Vigo

Department of Pharmaceutical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.

Original language	English
Title of host publication	Epithelial Cell Culture. Methods in Molecular Biology
Pages	89-93
Number of pages	5
Volume	1817
DOIs	https://doi.org/10.1007/978-1-4939-8600-2_9
Publication status	Published - 2018

Keywords

3D culture
Alginate
Encapsulation
Granulosa cells

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10.1007/978-1-4939-8600-2_9

http://hdl.handle.net/11579/144388

Cite this

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title = "Bioencapsulation of Oocytes and Granulosa Cells",

abstract = "A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.",

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T1 - Bioencapsulation of Oocytes and Granulosa Cells

AU - Faustini, Massimo

AU - Curone, Giulio

AU - TORRE, MARIA LUISA

AU - Vigo, Daniele

PY - 2018

Y1 - 2018

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AB - A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.

KW - 3D culture

KW - Alginate

KW - Encapsulation

KW - Granulosa cells

KW - 3D culture

KW - Alginate

KW - Encapsulation

KW - Granulosa cells

UR - https://iris.uniupo.it/handle/11579/144388

U2 - 10.1007/978-1-4939-8600-2_9

DO - 10.1007/978-1-4939-8600-2_9

M3 - Chapter

SN - 978-1-4939-8599-9

VL - 1817

SP - 89

EP - 93

BT - Epithelial Cell Culture. Methods in Molecular Biology

ER -

Bioencapsulation of Oocytes and Granulosa Cells

Abstract

Keywords

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