Biochemical characterization of a copper-thionein involved in Cu accumulation in the lysosomes of the digestive gland of mussels exposed to the metal

A. Viarengo; L. Canesi; M. Pertica; G. Mancinelli; M. Orunesu; A. Mazzucotelli; J. M. Bouquegneau

doi:10.1016/0141-1136(88)90286-3

Biochemical characterization of a copper-thionein involved in Cu accumulation in the lysosomes of the digestive gland of mussels exposed to the metal

A. Viarengo, L. Canesi, M. Pertica, G. Mancinelli, M. Orunesu, A. Mazzucotelli, J. M. Bouquegneau

Research output: Contribution to journal › Article › peer-review

Abstract

The Cu-rich protein present in an insoluble form in the digestive gland lysosomes of mussels (Mytilus galloprovincialis Lam.) exposed to sublethal concentrations of copper has been extracted and characterized. When solubilized, the Cu-protein has chromatographic and electrophoretic behaviour and a UV spectrum typical of cytosolic metallothioneins ofmussels. The amino acid composition is also similar to the cytosolic metallothioneins, from which it differs, for an unusually low glycine content and for a high level of aspartic and glutamic acid. Therefore, in mussel digestive gland metallothioneins and lysosomes may play an interrelated key role in copper homeostasis.

Original language	English
Pages (from-to)	163-166
Number of pages	4
Journal	Marine Environmental Research
Volume	24
Issue number	1-4
DOIs	https://doi.org/10.1016/0141-1136(88)90286-3
Publication status	Published - 1988
Externally published	Yes

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title = "Biochemical characterization of a copper-thionein involved in Cu accumulation in the lysosomes of the digestive gland of mussels exposed to the metal",

abstract = "The Cu-rich protein present in an insoluble form in the digestive gland lysosomes of mussels (Mytilus galloprovincialis Lam.) exposed to sublethal concentrations of copper has been extracted and characterized. When solubilized, the Cu-protein has chromatographic and electrophoretic behaviour and a UV spectrum typical of cytosolic metallothioneins ofmussels. The amino acid composition is also similar to the cytosolic metallothioneins, from which it differs, for an unusually low glycine content and for a high level of aspartic and glutamic acid. Therefore, in mussel digestive gland metallothioneins and lysosomes may play an interrelated key role in copper homeostasis.",

author = "A. Viarengo and L. Canesi and M. Pertica and G. Mancinelli and M. Orunesu and A. Mazzucotelli and Bouquegneau, {J. M.}",

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T1 - Biochemical characterization of a copper-thionein involved in Cu accumulation in the lysosomes of the digestive gland of mussels exposed to the metal

AU - Viarengo, A.

AU - Canesi, L.

AU - Pertica, M.

AU - Mancinelli, G.

AU - Orunesu, M.

AU - Mazzucotelli, A.

AU - Bouquegneau, J. M.

PY - 1988

Y1 - 1988

N2 - The Cu-rich protein present in an insoluble form in the digestive gland lysosomes of mussels (Mytilus galloprovincialis Lam.) exposed to sublethal concentrations of copper has been extracted and characterized. When solubilized, the Cu-protein has chromatographic and electrophoretic behaviour and a UV spectrum typical of cytosolic metallothioneins ofmussels. The amino acid composition is also similar to the cytosolic metallothioneins, from which it differs, for an unusually low glycine content and for a high level of aspartic and glutamic acid. Therefore, in mussel digestive gland metallothioneins and lysosomes may play an interrelated key role in copper homeostasis.

AB - The Cu-rich protein present in an insoluble form in the digestive gland lysosomes of mussels (Mytilus galloprovincialis Lam.) exposed to sublethal concentrations of copper has been extracted and characterized. When solubilized, the Cu-protein has chromatographic and electrophoretic behaviour and a UV spectrum typical of cytosolic metallothioneins ofmussels. The amino acid composition is also similar to the cytosolic metallothioneins, from which it differs, for an unusually low glycine content and for a high level of aspartic and glutamic acid. Therefore, in mussel digestive gland metallothioneins and lysosomes may play an interrelated key role in copper homeostasis.

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U2 - 10.1016/0141-1136(88)90286-3

DO - 10.1016/0141-1136(88)90286-3

M3 - Article

SN - 0141-1136

VL - 24

SP - 163

EP - 166

JO - Marine Environmental Research

JF - Marine Environmental Research

IS - 1-4

ER -

Biochemical characterization of a copper-thionein involved in Cu accumulation in the lysosomes of the digestive gland of mussels exposed to the metal

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