Bioactive glass functionalized with alkaline phosphatase stimulates bone extracellular matrix deposition and calcification in vitro

E. Vernè; S. Ferraris; C. Vitale-Brovarone; A. Cochis; L. Rimondini

doi:10.1016/j.apsusc.2014.06.001

Bioactive glass functionalized with alkaline phosphatase stimulates bone extracellular matrix deposition and calcification in vitro

E. Vernè
, S. Ferraris
, C. Vitale-Brovarone
, A. Cochis
, L. Rimondini

Department of Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Surface functionalization of bioactive glasses with the enzyme alkaline phosphatase (ALP) was studied in order to combine the inorganic properties of bioactive glass (bioactivity) with the biological properties of the biomolecule (mineralization). Two different bioactive glasses (SCNA and CEL2), with different degree of bioactivity, were used as substrates for the functionalization. ALP was grafted either by direct bonding or via an organic coupling agent. The cellular response to surface-modified bioactive glass was investigated using human osteosarcoma cells (MG63, osteoblast-like). Biocompatibility of all modified surfaces was verified. Of note, the more reactive glass (CEL2), modified with ALP, showed improved mineralization ability.

Original language	English
Pages (from-to)	372-381
Number of pages	10
Journal	Applied Surface Science
Volume	313
DOIs	https://doi.org/10.1016/j.apsusc.2014.06.001
Publication status	Published - 15 Sept 2014

Keywords

Alkaline phosphatase
Bioactive glasses
Osteoblasts
Surface functionalization

Access to Document

10.1016/j.apsusc.2014.06.001

Cite this

@article{a62a8f5fb4d147eb994ed5cb9ead1535,

title = "Bioactive glass functionalized with alkaline phosphatase stimulates bone extracellular matrix deposition and calcification in vitro",

abstract = "Surface functionalization of bioactive glasses with the enzyme alkaline phosphatase (ALP) was studied in order to combine the inorganic properties of bioactive glass (bioactivity) with the biological properties of the biomolecule (mineralization). Two different bioactive glasses (SCNA and CEL2), with different degree of bioactivity, were used as substrates for the functionalization. ALP was grafted either by direct bonding or via an organic coupling agent. The cellular response to surface-modified bioactive glass was investigated using human osteosarcoma cells (MG63, osteoblast-like). Biocompatibility of all modified surfaces was verified. Of note, the more reactive glass (CEL2), modified with ALP, showed improved mineralization ability.",

keywords = "Alkaline phosphatase, Bioactive glasses, Osteoblasts, Surface functionalization",

author = "E. Vern{\`e} and S. Ferraris and C. Vitale-Brovarone and A. Cochis and L. Rimondini",

year = "2014",

month = sep,

day = "15",

doi = "10.1016/j.apsusc.2014.06.001",

language = "English",

volume = "313",

pages = "372--381",

journal = "Applied Surface Science",

issn = "0169-4332",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Bioactive glass functionalized with alkaline phosphatase stimulates bone extracellular matrix deposition and calcification in vitro

AU - Vernè, E.

AU - Ferraris, S.

AU - Vitale-Brovarone, C.

AU - Cochis, A.

AU - Rimondini, L.

PY - 2014/9/15

Y1 - 2014/9/15

N2 - Surface functionalization of bioactive glasses with the enzyme alkaline phosphatase (ALP) was studied in order to combine the inorganic properties of bioactive glass (bioactivity) with the biological properties of the biomolecule (mineralization). Two different bioactive glasses (SCNA and CEL2), with different degree of bioactivity, were used as substrates for the functionalization. ALP was grafted either by direct bonding or via an organic coupling agent. The cellular response to surface-modified bioactive glass was investigated using human osteosarcoma cells (MG63, osteoblast-like). Biocompatibility of all modified surfaces was verified. Of note, the more reactive glass (CEL2), modified with ALP, showed improved mineralization ability.

AB - Surface functionalization of bioactive glasses with the enzyme alkaline phosphatase (ALP) was studied in order to combine the inorganic properties of bioactive glass (bioactivity) with the biological properties of the biomolecule (mineralization). Two different bioactive glasses (SCNA and CEL2), with different degree of bioactivity, were used as substrates for the functionalization. ALP was grafted either by direct bonding or via an organic coupling agent. The cellular response to surface-modified bioactive glass was investigated using human osteosarcoma cells (MG63, osteoblast-like). Biocompatibility of all modified surfaces was verified. Of note, the more reactive glass (CEL2), modified with ALP, showed improved mineralization ability.

KW - Alkaline phosphatase

KW - Bioactive glasses

KW - Osteoblasts

KW - Surface functionalization

UR - https://www.scopus.com/pages/publications/84904817357

U2 - 10.1016/j.apsusc.2014.06.001

DO - 10.1016/j.apsusc.2014.06.001

M3 - Article

SN - 0169-4332

VL - 313

SP - 372

EP - 381

JO - Applied Surface Science

JF - Applied Surface Science

ER -

Bioactive glass functionalized with alkaline phosphatase stimulates bone extracellular matrix deposition and calcification in vitro

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this