TY - JOUR
T1 - Behaviour of human mesenchymal stem cells on a polyelectrolyte-modified HEMA hydrogel for silk-based ligament tissue engineering
AU - BOSETTI, Michela
AU - BOCCAFOSCHI, FRANCESCA
AU - CALARCO, ANNA
AU - Leigheb, Massimiliano
AU - GATTI, Silvia
AU - Piffanelli, V
AU - Peluso, G
AU - CANNAS, Mario
N1 - Funding Information:
The authors are indebted to dr. G. Freddi, Stazione Sperimentale della Seta, Milano, who furnished the raw Bombyx mori silkworm fibres, of size 20/22, used in the experiments. This work was funded in part by Fondazione Cariplo, project “La ricostruzione legamentosa con uso di cellule staminali: studio sperimentale” (to M. C., contract no. 2002.1952/11.9974).
PY - 2008
Y1 - 2008
N2 - The aim of this study was to design a functional bio-engineered material to be used as scaffold for autologous mesenchymal stem cells in ligament tissue engineering. Polyelectrolyte modified HEMA hydrogel (HEMA-co-METAC), applied as coating on silk fibroin fibres, has been formulated in order to take advantage of the biocompatibility of the polyelectrolyte by increasing its mechanical properties with silk fibres. Human bone marrow mesenchymal stem cells behaviour on such reinforced polyelectrolyte has been studied by evaluating cell morphology, cell number, attachment, spreading and proliferation together with collagen matrix production and its mRNA expression. Silk fibroin fibres matrices with HEMA-co-METAC coating exhibited acceptable mechanical behaviour compared to the natural ligament, good human mesenchymal stem cell adhesion and with mRNA expression studies higher levels of collagen types I and III expression when compared to control cells on polystyrene. These data indicate high expression of mRNA for proteins responsible for the functional characteristics of the ligaments and suggest a potential for use of this biomaterial in ligament tissue-engineering applications.
AB - The aim of this study was to design a functional bio-engineered material to be used as scaffold for autologous mesenchymal stem cells in ligament tissue engineering. Polyelectrolyte modified HEMA hydrogel (HEMA-co-METAC), applied as coating on silk fibroin fibres, has been formulated in order to take advantage of the biocompatibility of the polyelectrolyte by increasing its mechanical properties with silk fibres. Human bone marrow mesenchymal stem cells behaviour on such reinforced polyelectrolyte has been studied by evaluating cell morphology, cell number, attachment, spreading and proliferation together with collagen matrix production and its mRNA expression. Silk fibroin fibres matrices with HEMA-co-METAC coating exhibited acceptable mechanical behaviour compared to the natural ligament, good human mesenchymal stem cell adhesion and with mRNA expression studies higher levels of collagen types I and III expression when compared to control cells on polystyrene. These data indicate high expression of mRNA for proteins responsible for the functional characteristics of the ligaments and suggest a potential for use of this biomaterial in ligament tissue-engineering applications.
UR - https://iris.uniupo.it/handle/11579/29292
U2 - 10.1163/156856208785540145
DO - 10.1163/156856208785540145
M3 - Article
SN - 0920-5063
VL - 19(9)
SP - 1111
EP - 1123
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
IS - 9
ER -