An expeditious, multi-gram isolation protocol for the ultrapotent SERCA inhibitor thapsigargin

Alberto Pagani; Federica Pollastro; Silvia Spera; Mauro Ballero; Olov Sterner; Giovanni Appendino

doi:10.1177/1934578x0700200604

An expeditious, multi-gram isolation protocol for the ultrapotent SERCA inhibitor thapsigargin

Alberto Pagani
, Federica Pollastro
, Silvia Spera
, Mauro Ballero
, Olov Sterner
, Giovanni Appendino

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A streamlined strategy was developed to obtain a mixture of thapsigargins from the fruits of Thapsia garganica L. The mixture was resolved by RP-silica gel flash chromatography to provide, after trituration with ether, 0.32% thapsigargin (la) as a white powder. A novel, complex phenylpropanoid (neohelmanthicin D, 2a) was also obtained, and its structure confirmed by semisynthesis.

Original language	English
Pages (from-to)	637-642
Number of pages	6
Journal	Natural Product Communications
Volume	2
Issue number	6
DOIs	https://doi.org/10.1177/1934578x0700200604
Publication status	Published - 2007

Keywords

Neohelmanthicin D
Serca
Thapsia garganica
Thapsigargicin
Thapsigargin
Thapsivillosin J

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10.1177/1934578x0700200604

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abstract = "A streamlined strategy was developed to obtain a mixture of thapsigargins from the fruits of Thapsia garganica L. The mixture was resolved by RP-silica gel flash chromatography to provide, after trituration with ether, 0.32\% thapsigargin (la) as a white powder. A novel, complex phenylpropanoid (neohelmanthicin D, 2a) was also obtained, and its structure confirmed by semisynthesis.",

keywords = "Neohelmanthicin D, Serca, Thapsia garganica, Thapsigargicin, Thapsigargin, Thapsivillosin J",

author = "Alberto Pagani and Federica Pollastro and Silvia Spera and Mauro Ballero and Olov Sterner and Giovanni Appendino",

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AU - Pagani, Alberto

AU - Pollastro, Federica

AU - Spera, Silvia

AU - Ballero, Mauro

AU - Sterner, Olov

AU - Appendino, Giovanni

PY - 2007

Y1 - 2007

N2 - A streamlined strategy was developed to obtain a mixture of thapsigargins from the fruits of Thapsia garganica L. The mixture was resolved by RP-silica gel flash chromatography to provide, after trituration with ether, 0.32% thapsigargin (la) as a white powder. A novel, complex phenylpropanoid (neohelmanthicin D, 2a) was also obtained, and its structure confirmed by semisynthesis.

AB - A streamlined strategy was developed to obtain a mixture of thapsigargins from the fruits of Thapsia garganica L. The mixture was resolved by RP-silica gel flash chromatography to provide, after trituration with ether, 0.32% thapsigargin (la) as a white powder. A novel, complex phenylpropanoid (neohelmanthicin D, 2a) was also obtained, and its structure confirmed by semisynthesis.

KW - Neohelmanthicin D

KW - Serca

KW - Thapsia garganica

KW - Thapsigargicin

KW - Thapsigargin

KW - Thapsivillosin J

UR - https://www.scopus.com/pages/publications/78549265885

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JO - Natural Product Communications

JF - Natural Product Communications

IS - 6

ER -

An expeditious, multi-gram isolation protocol for the ultrapotent SERCA inhibitor thapsigargin

Abstract

Keywords

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