Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

Raffaella Calligaris; Stefania Bottardi; Susanna Cogoi; Isabel Apezteguia; Claudio Santoro

doi:10.1073/pnas.92.25.11598

Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

Raffaella Calligaris
, Stefania Bottardi
, Susanna Cogoi
, Isabel Apezteguia
, Claudio Santoro

Research output: Contribution to journal › Article › peer-review

Abstract

GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.

Original language	English
Pages (from-to)	11598-11602
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	92
Issue number	25
DOIs	https://doi.org/10.1073/pnas.92.25.11598
Publication status	Published - 5 Dec 1995
Externally published	Yes

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10.1073/pnas.92.25.11598

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title = "Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor",

abstract = "GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.",

author = "Raffaella Calligaris and Stefania Bottardi and Susanna Cogoi and Isabel Apezteguia and Claudio Santoro",

year = "1995",

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doi = "10.1073/pnas.92.25.11598",

language = "English",

volume = "92",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor. / Calligaris, Raffaella; Bottardi, Stefania; Cogoi, Susanna et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 25, 05.12.1995, p. 11598-11602.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

AU - Calligaris, Raffaella

AU - Bottardi, Stefania

AU - Cogoi, Susanna

AU - Apezteguia, Isabel

AU - Santoro, Claudio

PY - 1995/12/5

Y1 - 1995/12/5

N2 - GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.

AB - GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.

UR - https://www.scopus.com/pages/publications/0029558618

U2 - 10.1073/pnas.92.25.11598

DO - 10.1073/pnas.92.25.11598

M3 - Article

SN - 0027-8424

VL - 92

SP - 11598

EP - 11602

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 25

ER -

Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

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