Allele frequencies for the PowerPlex™ 16 STR loci in an Albanian population sample from Northern Italy

C. Robino; S. Gino; C. Torre

doi:10.1520/jfs15086j

Allele frequencies for the PowerPlex^™ 16 STR loci in an Albanian population sample from Northern Italy

C. Robino
, S. Gino
, C. Torre

Research output: Contribution to journal › Article › peer-review

Abstract

In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm

Original language	English
Pages (from-to)	998-999
Number of pages	2
Journal	Journal of Forensic Sciences
Volume	46
Issue number	4
DOIs	https://doi.org/10.1520/jfs15086j
Publication status	Published - 2001
Externally published	Yes

Keywords

Albanians
CSF1PO
D13S317
D16S539
D18S51
D21S11
D3S1358
D5S818
D7S820
D8S1179
DNA typing
FGA
Forensic science
Italy
PENTA D
PENTA E
Polymerase chain reaction
Population genetics
Short tandem repeats
TH01
TPOX
VWA

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10.1520/jfs15086j

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title = "Allele frequencies for the PowerPlex{\texttrademark} 16 STR loci in an Albanian population sample from Northern Italy",

abstract = "In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint{\texttrademark} PowerPlex{\texttrademark} System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm",

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author = "C. Robino and S. Gino and C. Torre",

year = "2001",

doi = "10.1520/jfs15086j",

language = "English",

volume = "46",

pages = "998--999",

journal = "Journal of Forensic Sciences",

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AU - Robino, C.

AU - Gino, S.

AU - Torre, C.

PY - 2001

Y1 - 2001

N2 - In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm

AB - In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm

KW - Albanians

KW - CSF1PO

KW - D13S317

KW - D16S539

KW - D18S51

KW - D21S11

KW - D3S1358

KW - D5S818

KW - D7S820

KW - D8S1179

KW - DNA typing

KW - FGA

KW - Forensic science

KW - Italy

KW - PENTA D

KW - PENTA E

KW - Polymerase chain reaction

KW - Population genetics

KW - Short tandem repeats

KW - TH01

KW - TPOX

KW - VWA

UR - https://www.scopus.com/pages/publications/0034949267

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DO - 10.1520/jfs15086j

M3 - Article

SN - 0022-1198

VL - 46

SP - 998

EP - 999

JO - Journal of Forensic Sciences

JF - Journal of Forensic Sciences

IS - 4

ER -

Allele frequencies for the PowerPlex^™ 16 STR loci in an Albanian population sample from Northern Italy

Abstract

Keywords

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