Algorithms for warping of 2-D PAGE maps

MARCELLO MANFREDI; Elisa ROBOTTI; Emilio MARENGO

doi:10.1007/978-1-4939-3255-9_8

Algorithms for warping of 2-D PAGE maps

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Software-based image analysis of 2-D PAGE maps is an important step for the investigation of proteome. Warping algorithms, which are employed to register spots among gels, are able to overcome the difficulties due to the low reproducibility of this analytical technique. Over the years, the research of new matching and warping mathematical methods has allowed the development of several routine applications of easy-to-use software. This chapter describes common and basic spatial transformations used for the alignment of protein spots present in different gel maps; some recently new approaches are also presented.

Original language	English
Title of host publication	Methods in Molecular Biology: 2D-PAGE map analysis
Publisher	Springer New York
Pages	119-154
Number of pages	36
Volume	1384
ISBN (Print)	978-1-4939-3254-2
DOIs	https://doi.org/10.1007/978-1-4939-3255-9_8
Publication status	Published - 2016

Keywords

2-D PAGE analysis
Data analysis
Gel matching
Image processing
Warping

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10.1007/978-1-4939-3255-9_8

http://hdl.handle.net/11579/73392

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title = "Algorithms for warping of 2-D PAGE maps",

abstract = "Software-based image analysis of 2-D PAGE maps is an important step for the investigation of proteome. Warping algorithms, which are employed to register spots among gels, are able to overcome the difficulties due to the low reproducibility of this analytical technique. Over the years, the research of new matching and warping mathematical methods has allowed the development of several routine applications of easy-to-use software. This chapter describes common and basic spatial transformations used for the alignment of protein spots present in different gel maps; some recently new approaches are also presented.",

keywords = "2-D PAGE analysis, Data analysis, Gel matching, Image processing, Warping, 2-D PAGE analysis, Data analysis, Gel matching, Image processing, Warping",

author = "MARCELLO MANFREDI and Elisa ROBOTTI and Emilio MARENGO",

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AU - ROBOTTI, Elisa

AU - MARENGO, Emilio

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AB - Software-based image analysis of 2-D PAGE maps is an important step for the investigation of proteome. Warping algorithms, which are employed to register spots among gels, are able to overcome the difficulties due to the low reproducibility of this analytical technique. Over the years, the research of new matching and warping mathematical methods has allowed the development of several routine applications of easy-to-use software. This chapter describes common and basic spatial transformations used for the alignment of protein spots present in different gel maps; some recently new approaches are also presented.

KW - 2-D PAGE analysis

KW - Data analysis

KW - Gel matching

KW - Image processing

KW - Warping

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KW - Data analysis

KW - Gel matching

KW - Image processing

KW - Warping

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VL - 1384

SP - 119

EP - 154

BT - Methods in Molecular Biology: 2D-PAGE map analysis

PB - Springer New York

ER -

Algorithms for warping of 2-D PAGE maps

Abstract

Keywords

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