@inbook{6c56396bade64f5c87ad6379aacd94ce,
title = "Alcian Blue Staining to Visualize Intracellular Hyaluronic Acid-Based Nanoparticles",
abstract = "Investigating at transmission electron microscopy the intracellular trafficking of hyaluronic acid-based nanoparticles remains a challenge due to their intrinsic weak electron density. Here we describe a simple protocol to stain hyaluronic acid that allows visualization of hyaluronic acid-based nanoparticles inside cells at both light and electron microscopy. By applying the critical-electrolyte-concentration Alcian blue method, these nanoparticles were observed as blue dots at bright-field microscopy or filled with fine electron dense precipitates at transmission electron microscopy.",
keywords = "Histochemistry, Hyaluronate, Light microscopy, Nanocarriers, Transmission electron microscopy",
author = "Mathieu Repellin and Flavia Carton and Giovanna Lollo and Manuela Malatesta",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2675-7\_25",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "313--320",
booktitle = "Methods in Molecular Biology",
}