Actions of 3α,5α-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat

Rosa Ermirio; Donatella Blanchi; Piero Ruggeri; Carla E. Cogo; Claudio Molinari

doi:10.1016/0304-3940(89)90339-X

Actions of 3α,5α-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat

Rosa Ermirio
, Donatella Blanchi
, Piero Ruggeri
, Carla E. Cogo
, Claudio Molinari

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of microinjections of 3α,5α-tetrahydrodeoxycorticosterone (3α-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 μM 3α-THDOC inhibited firing of 105 of 112 neurones (94%). Microinjections of approximately 100 nl of 100-250 nM of 3α-THDOC did not alter neuronal activity, but in 52 of 68 cases (76%) it potentiated the inhibitory action of microiontophoretically applied γ-aminobutyric acid (GABA). The 3β-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3α-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3α-THDOC may function as endogenous modulator of GABA^A-mediated inhibition in various physiopathological conditions.

Original language	English
Pages (from-to)	115-120
Number of pages	6
Journal	Neuroscience Letters
Volume	104
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(89)90339-X
Publication status	Published - 25 Sept 1989
Externally published	Yes

Keywords

Brainstem
Microinjection
Rat
Reticular formation
Steroid hormone
Tetrahydrodeoxycorticosterone

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10.1016/0304-3940(89)90339-X

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title = "Actions of 3α,5α-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat",

abstract = "The effects of microinjections of 3α,5α-tetrahydrodeoxycorticosterone (3α-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 μM 3α-THDOC inhibited firing of 105 of 112 neurones (94\%). Microinjections of approximately 100 nl of 100-250 nM of 3α-THDOC did not alter neuronal activity, but in 52 of 68 cases (76\%) it potentiated the inhibitory action of microiontophoretically applied γ-aminobutyric acid (GABA). The 3β-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3α-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3α-THDOC may function as endogenous modulator of GABAA-mediated inhibition in various physiopathological conditions.",

keywords = "Brainstem, Microinjection, Rat, Reticular formation, Steroid hormone, Tetrahydrodeoxycorticosterone",

author = "Rosa Ermirio and Donatella Blanchi and Piero Ruggeri and Cogo, \{Carla E.\} and Claudio Molinari",

year = "1989",

month = sep,

day = "25",

doi = "10.1016/0304-3940(89)90339-X",

language = "English",

volume = "104",

pages = "115--120",

journal = "Neuroscience Letters",

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T1 - Actions of 3α,5α-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat

AU - Ermirio, Rosa

AU - Blanchi, Donatella

AU - Ruggeri, Piero

AU - Cogo, Carla E.

AU - Molinari, Claudio

PY - 1989/9/25

Y1 - 1989/9/25

N2 - The effects of microinjections of 3α,5α-tetrahydrodeoxycorticosterone (3α-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 μM 3α-THDOC inhibited firing of 105 of 112 neurones (94%). Microinjections of approximately 100 nl of 100-250 nM of 3α-THDOC did not alter neuronal activity, but in 52 of 68 cases (76%) it potentiated the inhibitory action of microiontophoretically applied γ-aminobutyric acid (GABA). The 3β-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3α-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3α-THDOC may function as endogenous modulator of GABAA-mediated inhibition in various physiopathological conditions.

AB - The effects of microinjections of 3α,5α-tetrahydrodeoxycorticosterone (3α-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 μM 3α-THDOC inhibited firing of 105 of 112 neurones (94%). Microinjections of approximately 100 nl of 100-250 nM of 3α-THDOC did not alter neuronal activity, but in 52 of 68 cases (76%) it potentiated the inhibitory action of microiontophoretically applied γ-aminobutyric acid (GABA). The 3β-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3α-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3α-THDOC may function as endogenous modulator of GABAA-mediated inhibition in various physiopathological conditions.

KW - Brainstem

KW - Microinjection

KW - Rat

KW - Reticular formation

KW - Steroid hormone

KW - Tetrahydrodeoxycorticosterone

UR - https://www.scopus.com/pages/publications/0024325826

U2 - 10.1016/0304-3940(89)90339-X

DO - 10.1016/0304-3940(89)90339-X

M3 - Article

SN - 0304-3940

VL - 104

SP - 115

EP - 120

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Actions of 3α,5α-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat

Abstract

Keywords

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