Action of chronic choline administration on behavior and on cholinergic and noradrenergic systems

Chronic administration of low doses (0.2-0.8 g/kg/day) of choline caused in the rat an increase of errors evaluated in the staircase maze after 20 days of interruption of daily training. An analogous pharmacological treatment caused no modification of the acetylcholine (ACh) and norepinephrine (NE) levels and no consistent modification of the density of muscarinic and α₁-adrenergic receptors. Only at higher doses (2.5 g/kg/day) did chronic administration (20 days) of choline cause in several sections of the CNS, an increase of ACh and NE levels and of the muscarinic receptor density. These observations indicate that only at high doses of choline are there consistent modifications of the central cholinergic systems, suggesting that the behavioral modifications observed at low doses of choline are not determined by an upregulation of the central cholinergic system.