Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

E. Bertino; D. Farinasso; M. Cavaletto; A. Coscia; G. Prandi; M. G. Giuffrida; S. Costa; C. Fabris; A. Conti

Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

E. Bertino, D. Farinasso, M. Cavaletto, A. Coscia, G. Prandi, M. G. Giuffrida, S. Costa, C. Fabris, A. Conti

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

Original language	English
Pages (from-to)	15-19
Number of pages	5
Journal	Acta Biomedica
Volume	68
Issue number	SUPPL. 1
Publication status	Published - 1997
Externally published	Yes

Keywords

Bovine β-lactoglobuim
Cow's milk proteins allergy
Cross-reaction
ELISA
Human milk

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements",

abstract = "Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.",

keywords = "Bovine β-lactoglobuim, Cow's milk proteins allergy, Cross-reaction, ELISA, Human milk",

author = "E. Bertino and D. Farinasso and M. Cavaletto and A. Coscia and G. Prandi and Giuffrida, {M. G.} and S. Costa and C. Fabris and A. Conti",

year = "1997",

language = "English",

volume = "68",

pages = "15--19",

journal = "Acta Biomedica",

issn = "0392-4203",

publisher = "Mattioli1885",

number = "SUPPL. 1",

}

TY - JOUR

T1 - Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

AU - Bertino, E.

AU - Farinasso, D.

AU - Cavaletto, M.

AU - Coscia, A.

AU - Prandi, G.

AU - Giuffrida, M. G.

AU - Costa, S.

AU - Fabris, C.

AU - Conti, A.

PY - 1997

Y1 - 1997

N2 - Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

AB - Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

KW - Bovine β-lactoglobuim

KW - Cow's milk proteins allergy

KW - Cross-reaction

KW - ELISA

KW - Human milk

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M3 - Article

SN - 0392-4203

VL - 68

SP - 15

EP - 19

JO - Acta Biomedica

JF - Acta Biomedica

IS - SUPPL. 1

ER -

Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

Abstract

Keywords

UN SDGs

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