A VINCRISTINE-RESISTANT MURINE ERYTHROLEUKEMIA CELL-LINE SECRETES - A DIFFERENTIATION ENHANCING FACTOR

B SPARATORE; Mauro PATRONE; F. SALAMINO

doi:10.1016/S0006-291X(05)81035-6

A VINCRISTINE-RESISTANT MURINE ERYTHROLEUKEMIA CELL-LINE SECRETES - A DIFFERENTIATION ENHANCING FACTOR

B SPARATORE
, Mauro PATRONE
, F. SALAMINO

Department of Science and Technological Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

A clone of vincristine resistant murine erythroleukemia cells V3.17[44], characterized by high sensitivity to terminal erythroid differenitation induced by hexamethylene bisacetamide, secretes into the extracellular medium a protein factor which partially reduces the latent period before commitment and accelerates the expression of the terminal differentiated phenotype in a slow responding murine erythroleukemia N23 cell variant. This differentiation enhancing factor increases the rate of protein kinase C down-regulation which occurs at slower rate during cell differentiation. The activity of the factor is detected either by co-culture of the two cell line variants or by addition of conditioned medium from V3.17[44] cells to a culture of N23 cells in the presence of the inducer. In addition to being secreted by V3.17[44] cells, this factor can also be detected in the cytoplasm of both V3.17[44] and N23 cells, associated with a particulate fraction from which it can be released by sonication.

Original language	English
Pages (from-to)	156-163
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	173
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(05)81035-6
Publication status	Published - 1990

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10.1016/S0006-291X(05)81035-6

http://hdl.handle.net/11579/1674

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@article{da4854b4efc24f17901988f172538278,

title = "A VINCRISTINE-RESISTANT MURINE ERYTHROLEUKEMIA CELL-LINE SECRETES - A DIFFERENTIATION ENHANCING FACTOR",

abstract = "A clone of vincristine resistant murine erythroleukemia cells V3.17[44], characterized by high sensitivity to terminal erythroid differenitation induced by hexamethylene bisacetamide, secretes into the extracellular medium a protein factor which partially reduces the latent period before commitment and accelerates the expression of the terminal differentiated phenotype in a slow responding murine erythroleukemia N23 cell variant. This differentiation enhancing factor increases the rate of protein kinase C down-regulation which occurs at slower rate during cell differentiation. The activity of the factor is detected either by co-culture of the two cell line variants or by addition of conditioned medium from V3.17[44] cells to a culture of N23 cells in the presence of the inducer. In addition to being secreted by V3.17[44] cells, this factor can also be detected in the cytoplasm of both V3.17[44] and N23 cells, associated with a particulate fraction from which it can be released by sonication.",

author = "B SPARATORE and Mauro PATRONE and F. SALAMINO",

note = "Funding Information: We thank Dr.P.A. Marks and Dr.R.A. Rifkind for critically reviewing the manuscript. This work was supported by grants from the Consiglio Nazionale delle Ricerche, Progetto Strategico Prodotti Biotecnologici per il Controllo della Comunicazione Cellulare, and Progetto Finalizzato Biotecnologie e Biostrumenta-zione and by the Associazione Italiana per la Ricerca sul Cancro.",

year = "1990",

doi = "10.1016/S0006-291X(05)81035-6",

language = "English",

volume = "173",

pages = "156--163",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - A VINCRISTINE-RESISTANT MURINE ERYTHROLEUKEMIA CELL-LINE SECRETES - A DIFFERENTIATION ENHANCING FACTOR

AU - SPARATORE, B

AU - PATRONE, Mauro

AU - SALAMINO, F.

N1 - Funding Information: We thank Dr.P.A. Marks and Dr.R.A. Rifkind for critically reviewing the manuscript. This work was supported by grants from the Consiglio Nazionale delle Ricerche, Progetto Strategico Prodotti Biotecnologici per il Controllo della Comunicazione Cellulare, and Progetto Finalizzato Biotecnologie e Biostrumenta-zione and by the Associazione Italiana per la Ricerca sul Cancro.

PY - 1990

Y1 - 1990

N2 - A clone of vincristine resistant murine erythroleukemia cells V3.17[44], characterized by high sensitivity to terminal erythroid differenitation induced by hexamethylene bisacetamide, secretes into the extracellular medium a protein factor which partially reduces the latent period before commitment and accelerates the expression of the terminal differentiated phenotype in a slow responding murine erythroleukemia N23 cell variant. This differentiation enhancing factor increases the rate of protein kinase C down-regulation which occurs at slower rate during cell differentiation. The activity of the factor is detected either by co-culture of the two cell line variants or by addition of conditioned medium from V3.17[44] cells to a culture of N23 cells in the presence of the inducer. In addition to being secreted by V3.17[44] cells, this factor can also be detected in the cytoplasm of both V3.17[44] and N23 cells, associated with a particulate fraction from which it can be released by sonication.

AB - A clone of vincristine resistant murine erythroleukemia cells V3.17[44], characterized by high sensitivity to terminal erythroid differenitation induced by hexamethylene bisacetamide, secretes into the extracellular medium a protein factor which partially reduces the latent period before commitment and accelerates the expression of the terminal differentiated phenotype in a slow responding murine erythroleukemia N23 cell variant. This differentiation enhancing factor increases the rate of protein kinase C down-regulation which occurs at slower rate during cell differentiation. The activity of the factor is detected either by co-culture of the two cell line variants or by addition of conditioned medium from V3.17[44] cells to a culture of N23 cells in the presence of the inducer. In addition to being secreted by V3.17[44] cells, this factor can also be detected in the cytoplasm of both V3.17[44] and N23 cells, associated with a particulate fraction from which it can be released by sonication.

UR - https://iris.uniupo.it/handle/11579/1674

U2 - 10.1016/S0006-291X(05)81035-6

DO - 10.1016/S0006-291X(05)81035-6

M3 - Article

SN - 0006-291X

VL - 173

SP - 156

EP - 163

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

A VINCRISTINE-RESISTANT MURINE ERYTHROLEUKEMIA CELL-LINE SECRETES - A DIFFERENTIATION ENHANCING FACTOR

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