A UPLC-MS-MS method for the simultaneous quantification of first-line antituberculars in plasma and in PBMCs

LORENA BAIETTO; Andrea CALCAGNO; ILARIA MOTTA; Katia Baruffi; Viviana Poretti; Giovanni DI PERRI; Stefano BONORA; ANTONIO D'AVOLIO

doi:10.1093/jac/dkv148

A UPLC-MS-MS method for the simultaneous quantification of first-line antituberculars in plasma and in PBMCs

LORENA BAIETTO
, Andrea CALCAGNO
, ILARIA MOTTA
, Katia Baruffi
, Viviana Poretti
, Giovanni DI PERRI
, Stefano BONORA
, ANTONIO D'AVOLIO

Department of Translational Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

TB is currently the second cause of death among patients affected with infectious diseases. Quantification of drug levels in plasma and in cells where Mycobacterium tuberculosis persists and grows may be useful in understanding the appropriateness of dosage regimens. We report a new and fully validated chromatographic method to quantify first-line antituberculars in plasma and PBMCs. The method was used for plasma and cell quantification of antituberculars in patients undergoing treatment with standard oral therapy.

Original language	English
Pages (from-to)	2572-2575
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	70
Issue number	9
DOIs	https://doi.org/10.1093/jac/dkv148
Publication status	Published - 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

HPLC
TDM
determination
ethambutol
isoniazid
peripheral blood mononuclear cells
pyrazinamide
rifampicin
therapeutic drug monitoring

Access to Document

10.1093/jac/dkv148

https://hdl.handle.net/11579/216906

Cite this

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title = "A UPLC-MS-MS method for the simultaneous quantification of first-line antituberculars in plasma and in PBMCs",

abstract = "TB is currently the second cause of death among patients affected with infectious diseases. Quantification of drug levels in plasma and in cells where Mycobacterium tuberculosis persists and grows may be useful in understanding the appropriateness of dosage regimens. We report a new and fully validated chromatographic method to quantify first-line antituberculars in plasma and PBMCs. The method was used for plasma and cell quantification of antituberculars in patients undergoing treatment with standard oral therapy.",

keywords = "HPLC, TDM, determination, ethambutol, isoniazid, peripheral blood mononuclear cells, pyrazinamide, rifampicin, therapeutic drug monitoring, HPLC, TDM, determination, ethambutol, isoniazid, peripheral blood mononuclear cells, pyrazinamide, rifampicin, therapeutic drug monitoring",

author = "LORENA BAIETTO and Andrea CALCAGNO and ILARIA MOTTA and Katia Baruffi and Viviana Poretti and \{DI PERRI\}, Giovanni and Stefano BONORA and ANTONIO D'AVOLIO",

year = "2015",

doi = "10.1093/jac/dkv148",

language = "English",

volume = "70",

pages = "2572--2575",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "9",

}

TY - JOUR

T1 - A UPLC-MS-MS method for the simultaneous quantification of first-line antituberculars in plasma and in PBMCs

AU - BAIETTO, LORENA

AU - CALCAGNO, Andrea

AU - MOTTA, ILARIA

AU - Baruffi, Katia

AU - Poretti, Viviana

AU - DI PERRI, Giovanni

AU - BONORA, Stefano

AU - D'AVOLIO, ANTONIO

PY - 2015

Y1 - 2015

N2 - TB is currently the second cause of death among patients affected with infectious diseases. Quantification of drug levels in plasma and in cells where Mycobacterium tuberculosis persists and grows may be useful in understanding the appropriateness of dosage regimens. We report a new and fully validated chromatographic method to quantify first-line antituberculars in plasma and PBMCs. The method was used for plasma and cell quantification of antituberculars in patients undergoing treatment with standard oral therapy.

AB - TB is currently the second cause of death among patients affected with infectious diseases. Quantification of drug levels in plasma and in cells where Mycobacterium tuberculosis persists and grows may be useful in understanding the appropriateness of dosage regimens. We report a new and fully validated chromatographic method to quantify first-line antituberculars in plasma and PBMCs. The method was used for plasma and cell quantification of antituberculars in patients undergoing treatment with standard oral therapy.

KW - HPLC

KW - TDM

KW - determination

KW - ethambutol

KW - isoniazid

KW - peripheral blood mononuclear cells

KW - pyrazinamide

KW - rifampicin

KW - therapeutic drug monitoring

KW - HPLC

KW - TDM

KW - determination

KW - ethambutol

KW - isoniazid

KW - peripheral blood mononuclear cells

KW - pyrazinamide

KW - rifampicin

KW - therapeutic drug monitoring

UR - https://iris.uniupo.it/handle/11579/216906

U2 - 10.1093/jac/dkv148

DO - 10.1093/jac/dkv148

M3 - Article

SN - 0305-7453

VL - 70

SP - 2572

EP - 2575

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 9

ER -

A UPLC-MS-MS method for the simultaneous quantification of first-line antituberculars in plasma and in PBMCs

Abstract

UN SDGs

Keywords

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