A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

R. Accomando; A. Viarengo; R. Bordone; M. Taningher; L. Canesi; M. Orunesu

doi:10.1016/0020-711X(91)90194-R

A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

R. Accomando
, A. Viarengo
, R. Bordone
, M. Taningher
, L. Canesi
, M. Orunesu

Research output: Contribution to journal › Article › peer-review

Abstract

1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.

Original language	English
Pages (from-to)	227-229
Number of pages	3
Journal	International Journal of Biochemistry
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0020-711X(91)90194-R
Publication status	Published - 1991
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

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10.1016/0020-711X(91)90194-R

Cite this

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title = "A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals",

abstract = "1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.",

author = "R. Accomando and A. Viarengo and R. Bordone and M. Taningher and L. Canesi and M. Orunesu",

year = "1991",

doi = "10.1016/0020-711X(91)90194-R",

language = "English",

volume = "23",

pages = "227--229",

journal = "International Journal of Biochemistry",

issn = "0020-711X",

publisher = "Pergamon Press Ltd.",

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TY - JOUR

T1 - A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

AU - Accomando, R.

AU - Viarengo, A.

AU - Bordone, R.

AU - Taningher, M.

AU - Canesi, L.

AU - Orunesu, M.

PY - 1991

Y1 - 1991

N2 - 1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.

AB - 1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.

UR - https://www.scopus.com/pages/publications/0026057031

U2 - 10.1016/0020-711X(91)90194-R

DO - 10.1016/0020-711X(91)90194-R

M3 - Article

SN - 0020-711X

VL - 23

SP - 227

EP - 229

JO - International Journal of Biochemistry

JF - International Journal of Biochemistry

IS - 2

ER -

A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

Abstract

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