A Pulsed Electric Field (PEF) bench static system to study bacteria inactivation

Pietro Cortese; Giuseppe Dellacasa; Roberto Gemme; Sara Bonetta; Silvia Bonetta; Elisabetta Carraro; Francesca Motta; Marco Paganoni; Marco Pizzichemi

doi:10.1016/j.nuclphysbps.2011.03.165

A Pulsed Electric Field (PEF) bench static system to study bacteria inactivation

Pietro Cortese
, Giuseppe Dellacasa
, Roberto Gemme
, Sara Bonetta
, Silvia Bonetta
, Elisabetta Carraro
, Francesca Motta
, Marco Paganoni
, Marco Pizzichemi

Department of Science and Technological Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

Pulsed Electric Fields (PEF) technology is a promising non-thermal processing method for inactivation of microorganisms. A small PEF bench system able to treat a 0.4 ml static liquid volume has been built and tested at the laboratories of the Università del Piemonte Orientale in Alessandria, Italy. The technique used to produce the required fields consists of charging high voltage cables of various lengths and subsequently discharge them on a cylindrical cell. The pulse intensity can be adjusted to reach a maximum electric field in the cell of about 35 kV/cm and the pulse frequency can reach 10 Hz. We describe the PEF system in some detail and, as a benchmark of its performances, we report preliminary results obtained on Escherichia coli (ATCC 25922) at 10⁹ Cfu/ml concentration suspended in a McIlvaine buffer (pH 7.2).

Original language	English
Pages (from-to)	162-164
Number of pages	3
Journal	Nuclear Physics B - Proceedings Supplements
Volume	215
Issue number	1
DOIs	https://doi.org/10.1016/j.nuclphysbps.2011.03.165
Publication status	Published - Jun 2011

Access to Document

10.1016/j.nuclphysbps.2011.03.165

Cite this

@article{1a8c01da01274355884557fea22d0ba3,

title = "A Pulsed Electric Field (PEF) bench static system to study bacteria inactivation",

abstract = "Pulsed Electric Fields (PEF) technology is a promising non-thermal processing method for inactivation of microorganisms. A small PEF bench system able to treat a 0.4 ml static liquid volume has been built and tested at the laboratories of the Universit{\`a} del Piemonte Orientale in Alessandria, Italy. The technique used to produce the required fields consists of charging high voltage cables of various lengths and subsequently discharge them on a cylindrical cell. The pulse intensity can be adjusted to reach a maximum electric field in the cell of about 35 kV/cm and the pulse frequency can reach 10 Hz. We describe the PEF system in some detail and, as a benchmark of its performances, we report preliminary results obtained on Escherichia coli (ATCC 25922) at 109 Cfu/ml concentration suspended in a McIlvaine buffer (pH 7.2).",

author = "Pietro Cortese and Giuseppe Dellacasa and Roberto Gemme and Sara Bonetta and Silvia Bonetta and Elisabetta Carraro and Francesca Motta and Marco Paganoni and Marco Pizzichemi",

note = "Funding Information: We would like to thank Silvano Gallian of the INFN section of Torino-Italy who took a great and irreplaceable part in designing and building the apparatus. We deeply acknowledge Dr. Cesare Ponti of the PONTI S.p.A. for providing us with the Samtech trigger generator TG-01. We are indebted to Dr. Peter Pearce and to the TERA foundation which took part in the early stage of the project. Discussions with Prof. Gio-vanna Ferrari and Ing. Gian Piero Pataro of the Salerno University - Italy have been very fruitful. The project has been totally funded by the Piedmont Region (Italy) in the frame of the CIPE 2007 program in the section Food Security.",

year = "2011",

month = jun,

doi = "10.1016/j.nuclphysbps.2011.03.165",

language = "English",

volume = "215",

pages = "162--164",

journal = "Nuclear Physics B - Proceedings Supplements",

issn = "0920-5632",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - A Pulsed Electric Field (PEF) bench static system to study bacteria inactivation

AU - Cortese, Pietro

AU - Dellacasa, Giuseppe

AU - Gemme, Roberto

AU - Bonetta, Sara

AU - Bonetta, Silvia

AU - Carraro, Elisabetta

AU - Motta, Francesca

AU - Paganoni, Marco

AU - Pizzichemi, Marco

N1 - Funding Information: We would like to thank Silvano Gallian of the INFN section of Torino-Italy who took a great and irreplaceable part in designing and building the apparatus. We deeply acknowledge Dr. Cesare Ponti of the PONTI S.p.A. for providing us with the Samtech trigger generator TG-01. We are indebted to Dr. Peter Pearce and to the TERA foundation which took part in the early stage of the project. Discussions with Prof. Gio-vanna Ferrari and Ing. Gian Piero Pataro of the Salerno University - Italy have been very fruitful. The project has been totally funded by the Piedmont Region (Italy) in the frame of the CIPE 2007 program in the section Food Security.

PY - 2011/6

Y1 - 2011/6

N2 - Pulsed Electric Fields (PEF) technology is a promising non-thermal processing method for inactivation of microorganisms. A small PEF bench system able to treat a 0.4 ml static liquid volume has been built and tested at the laboratories of the Università del Piemonte Orientale in Alessandria, Italy. The technique used to produce the required fields consists of charging high voltage cables of various lengths and subsequently discharge them on a cylindrical cell. The pulse intensity can be adjusted to reach a maximum electric field in the cell of about 35 kV/cm and the pulse frequency can reach 10 Hz. We describe the PEF system in some detail and, as a benchmark of its performances, we report preliminary results obtained on Escherichia coli (ATCC 25922) at 109 Cfu/ml concentration suspended in a McIlvaine buffer (pH 7.2).

AB - Pulsed Electric Fields (PEF) technology is a promising non-thermal processing method for inactivation of microorganisms. A small PEF bench system able to treat a 0.4 ml static liquid volume has been built and tested at the laboratories of the Università del Piemonte Orientale in Alessandria, Italy. The technique used to produce the required fields consists of charging high voltage cables of various lengths and subsequently discharge them on a cylindrical cell. The pulse intensity can be adjusted to reach a maximum electric field in the cell of about 35 kV/cm and the pulse frequency can reach 10 Hz. We describe the PEF system in some detail and, as a benchmark of its performances, we report preliminary results obtained on Escherichia coli (ATCC 25922) at 109 Cfu/ml concentration suspended in a McIlvaine buffer (pH 7.2).

UR - https://www.scopus.com/pages/publications/79958290855

U2 - 10.1016/j.nuclphysbps.2011.03.165

DO - 10.1016/j.nuclphysbps.2011.03.165

M3 - Article

SN - 0920-5632

VL - 215

SP - 162

EP - 164

JO - Nuclear Physics B - Proceedings Supplements

JF - Nuclear Physics B - Proceedings Supplements

IS - 1

ER -

A Pulsed Electric Field (PEF) bench static system to study bacteria inactivation

Abstract

Access to Document

Other files and links

Fingerprint

Cite this